Marker-independent identification of glioma-initiating cells.

Détails

ID Serval
serval:BIB_91555CC6F3C8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Marker-independent identification of glioma-initiating cells.
Périodique
Nature methods
Auteur(s)
Clément V., Marino D., Cudalbu C., Hamou M.F., Mlynarik V., de Tribolet N., Dietrich P.Y., Gruetter R., Hegi M.E., Radovanovic I.
ISSN
1548-7105 (Electronic)
ISSN-L
1548-7091
Statut éditorial
Publié
Date de publication
03/2010
Peer-reviewed
Oui
Volume
7
Numéro
3
Pages
224-228
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication
Publication Status: ppublish
Résumé
Tumor-initiating cells with stem cell properties are believed to sustain the growth of gliomas, but proposed markers such as CD133 cannot be used to identify these cells with sufficient specificity. We report an alternative isolation method purely based on phenotypic qualities of glioma-initiating cells (GICs), avoiding the use of molecular markers. We exploited intrinsic autofluorescence properties and a distinctive morphology to isolate a subpopulation of cells (FL1(+)) from human glioma or glioma cultures. FL1(+) cells are capable of self-renewal in vitro, tumorigenesis in vivo and preferentially express stem cell genes. The FL1(+) phenotype did not correlate with the expression of proposed GIC markers. Our data propose an alternative approach to investigate tumor-initiating potential in gliomas and to advance the development of new therapies and diagnostics.

Mots-clé
AC133 Antigen, Animals, Antigens, CD/analysis, Biomarkers, Tumor/analysis, Brain Neoplasms/pathology, Cell Differentiation, Cells, Cultured, Fluorescence, Gene Expression Profiling, Glioma/pathology, Glycoproteins/analysis, Humans, Mice, Neoplastic Stem Cells/pathology, Peptides/analysis
Pubmed
Web of science
Création de la notice
16/03/2010 14:52
Dernière modification de la notice
03/03/2018 19:28
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