Actin cytoskeleton organization regulated by the PAK family of protein kinases.

Détails

ID Serval
serval:BIB_9145
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Actin cytoskeleton organization regulated by the PAK family of protein kinases.
Périodique
Current Biology
Auteur(s)
Eby J.J., Holly S.P., van Drogen F., Grishin A.V., Peter M., Drubin D.G., Blumer K.J.
ISSN
0960-9822
Statut éditorial
Publié
Date de publication
1998
Volume
8
Numéro
17
Pages
967-970
Langue
anglais
Résumé
Cdc42, Rac1 and other Rho-type GTPases regulate gene expression, cell proliferation and cytoskeletal architecture [1,2]. A challenge is to identify the effectors of Cdc42 and Rac1 that mediate these biological responses. Protein kinases of the p21-activated kinase (PAK) family bind activated Rac1 and Cdc42, and switch on mitogen-activated protein (MAP) kinase pathways; however, their roles in regulating actin cytoskeleton organization have not been clearly established [3-5]. Here, we show that mutants of the budding yeast Saccharomyces cerevisiae lacking the PAK homologs Ste20 and Cla4 exhibit actin cytoskeletal defects, in vivo and in vitro, that resemble those of cdc42-1 mutants. Moreover, STE20 overexpression suppresses cdc42-1 growth defects and cytoskeletal defects in vivo, and Ste20 kinase corrects the actin-assembly defects of permeabilized cdc42-1 cells in vitro. Thus, PAKs are effectors of Cdc42 in pathways that regulate the organization of the cortical actin cytoskeleton.
Mots-clé
Actins/metabolism, Calcium-Calmodulin-Dependent Protein Kinases/physiology, Cell Cycle Proteins/genetics, Cell Cycle Proteins/physiology, Cell Polarity, Cytoskeleton/enzymology, Cytoskeleton/metabolism, GTP-Binding Proteins/genetics, GTP-Binding Proteins/physiology, Mutation, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/physiology, Recombinant Proteins, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae Proteins, Signal Transduction, Temperature, cdc42 GTP-Binding Protein, Saccharomyces cerevisiae
Pubmed
Web of science
Création de la notice
19/11/2007 13:47
Dernière modification de la notice
03/03/2018 19:28
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