Measurement of low angiotensin concentrations after ethanol and Dowex extraction procedures

Détails

ID Serval
serval:BIB_9138A29B7BA8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Measurement of low angiotensin concentrations after ethanol and Dowex extraction procedures
Périodique
Journal of Laboratory and Clinical Medicine
Auteur(s)
Nussberger  J., Brunner  D. B., Waeber  B., Brunner  H. R.
ISSN
0022-2143 (Print)
Statut éditorial
Publié
Date de publication
02/1984
Volume
103
Numéro
2
Pages
304-12
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
Current radioimmunoassays do not demonstrate total absence of angiotensin II during converting enzyme inhibition. To assess the meaning of plasma angiotensin II determinations during converting enzyme inhibition, plasma angiotensin I and II levels of normotensive humans during maximal converting enzyme inhibition by single oral doses of CGS 13945, MK 421, or MK 521 were compared with those of anephric rats (18 hr after nephrectomy) after intravenous administration of MK 422 (1 mg/kg). Prior to radioimmunoassay, plasma was extracted with Dowex for angiotensin II and blood extracted with ethanol for angiotensin I. During converting enzyme inhibition, in the 20 normotensive subjects plasma angiotensin II was 6.3 +/- 2.3 pg/ml (mean +/- S.D.) and blood angiotensin I was 65 +/- 59 pg/ml. In the nephrectomized rats, plasma angiotensin II was 8.9 +/- 2.3 pg/ml without converting enzyme inhibitor (n = 15) and 7.6 +/- 2.8 with MK 422 (n = 14), and blood angiotensin I was 9.8 +/- 2.4 pg/ml and 8.2 +/- 0.7, respectively. Dowex extraction of Tris buffer containing no angiotensin II provided blank values ranging from 5.0 to 7.8 pg/ml (n = 5). Thus plasma angiotensin II of normotensive humans treated with converting enzyme inhibitors fell to blank levels even in the presence of markedly elevated plasma angiotensin I. Angiotensin II concentrations in anephric rats with or without converting enzyme inhibition were the same. We therefore conclude that plasma levels of angiotensin II below 8 pg/ml measured after Dowex extraction probably reflect complete converting enzyme inhibition and virtual absence of angiotensin II generation.
Mots-clé
Adult Angiotensin I/*blood Angiotensin II/*blood Angiotensin-Converting Enzyme Inhibitors Angiotensins/*blood Animals Anion Exchange Resins/diagnostic use Dipeptides/pharmacology Enalapril Ethanol/diagnostic use Humans Indoles/pharmacology Kidney/physiology Lisinopril Male Radioimmunoassay Rats Resins, Synthetic
Pubmed
Web of science
Création de la notice
05/03/2008 17:40
Dernière modification de la notice
03/03/2018 19:27
Données d'usage