Differential reactivity of TCR Vbeta10 alleles to a mouse mammary tumor virus superantigen.

Details

Serval ID
serval:BIB_9112C47D0CA4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential reactivity of TCR Vbeta10 alleles to a mouse mammary tumor virus superantigen.
Journal
European Journal of Immunology
Author(s)
Maillard I., Xenarios I., Diggelmann H., Orbea H.A.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Publication state
Published
Issued date
1998
Peer-reviewed
Oui
Volume
28
Number
10
Pages
3075-3085
Language
english
Abstract
Mouse mammary tumor virus (MMTV) expresses a superantigen (SAg) which plays a critical role in the viral life cycle. We have recently described the new infectious MMTV (SIM) encoding a Vbeta4-specific SAg in mice with a TCR-Vbeta(b) haplotype. We have now compared the SAg activity of this virus in BALB/c mice harboring the TCR-Vbeta(a), TCR-Vbeta(b) or TCR-Vbeta(c) haplotypes which differ by a central deletion in the TCR-Vbeta(a) and TCR-Vbeta(c) locus and by mutations in some of the remaining Vbeta elements. Injection of MMTV (SIM) led to a strong stimulation of Vbeta4+ CD4+ T cells in TCR-Vbeta(b) mice, but only to a weak stimulation of these cells in TCR-Vbeta(a) or TCR-Vbeta(c) mice. A large increase in the percentage of Vbeta10+ cells was observed among CD4+ T cells in mice with the Vbeta(a) or Vbeta(c), but not the Vbeta(b) TCR-Vbeta haplotype. Vbeta10+ cells dominated the response when Vbeta10(a/c) and Vbeta4 subsets were present together. This is the first report of a viral SAg interacting with murine Vbeta10+ cells. Six amino acid differences between Vbeta10(a/c) and Vbeta10(b) could account for the gain of reactivity of Vbeta10(a/c) to the MMTV(SIM) SAg. No mutations were found in the hypervariable region 4 (HV4) of the TCR. Mutations at positions 22 and 28 introduce into Vbeta10(a/c) the same amino acids which are found at these positions in the MMTV(SIM)-reactive Vbeta4. Tridimensional models indicated that these amino acids lie close to HV4 and are likely to be important for the interaction of the SAg with the TCR.
Keywords
Alleles, Amino Acid Sequence, Animals, Antigens, Viral/immunology, Haplotypes, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Protein Conformation, Receptors, Antigen, T-Cell, alpha-beta/chemistry, Receptors, Antigen, T-Cell, alpha-beta/genetics, Superantigens/immunology
Pubmed
Web of science
Open Access
Yes
Create date
22/02/2008 17:33
Last modification date
20/08/2019 14:54
Usage data