Differential reactivity of TCR Vbeta10 alleles to a mouse mammary tumor virus superantigen.

Détails

ID Serval
serval:BIB_9112C47D0CA4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Differential reactivity of TCR Vbeta10 alleles to a mouse mammary tumor virus superantigen.
Périodique
European Journal of Immunology
Auteur(s)
Maillard I., Xenarios I., Diggelmann H., Orbea H.A.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1998
Peer-reviewed
Oui
Volume
28
Numéro
10
Pages
3075-3085
Langue
anglais
Résumé
Mouse mammary tumor virus (MMTV) expresses a superantigen (SAg) which plays a critical role in the viral life cycle. We have recently described the new infectious MMTV (SIM) encoding a Vbeta4-specific SAg in mice with a TCR-Vbeta(b) haplotype. We have now compared the SAg activity of this virus in BALB/c mice harboring the TCR-Vbeta(a), TCR-Vbeta(b) or TCR-Vbeta(c) haplotypes which differ by a central deletion in the TCR-Vbeta(a) and TCR-Vbeta(c) locus and by mutations in some of the remaining Vbeta elements. Injection of MMTV (SIM) led to a strong stimulation of Vbeta4+ CD4+ T cells in TCR-Vbeta(b) mice, but only to a weak stimulation of these cells in TCR-Vbeta(a) or TCR-Vbeta(c) mice. A large increase in the percentage of Vbeta10+ cells was observed among CD4+ T cells in mice with the Vbeta(a) or Vbeta(c), but not the Vbeta(b) TCR-Vbeta haplotype. Vbeta10+ cells dominated the response when Vbeta10(a/c) and Vbeta4 subsets were present together. This is the first report of a viral SAg interacting with murine Vbeta10+ cells. Six amino acid differences between Vbeta10(a/c) and Vbeta10(b) could account for the gain of reactivity of Vbeta10(a/c) to the MMTV(SIM) SAg. No mutations were found in the hypervariable region 4 (HV4) of the TCR. Mutations at positions 22 and 28 introduce into Vbeta10(a/c) the same amino acids which are found at these positions in the MMTV(SIM)-reactive Vbeta4. Tridimensional models indicated that these amino acids lie close to HV4 and are likely to be important for the interaction of the SAg with the TCR.
Mots-clé
Alleles, Amino Acid Sequence, Animals, Antigens, Viral/immunology, Haplotypes, Mammary Tumor Virus, Mouse/immunology, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Protein Conformation, Receptors, Antigen, T-Cell, alpha-beta/chemistry, Receptors, Antigen, T-Cell, alpha-beta/genetics, Superantigens/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/02/2008 17:33
Dernière modification de la notice
20/08/2019 14:54
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