Generation of cytotoxic T lymphocytes in vitro. VII. Suppressive effect of irradiated MLC cells on CTL response

Détails

ID Serval
serval:BIB_904E5FE14A0B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Generation of cytotoxic T lymphocytes in vitro. VII. Suppressive effect of irradiated MLC cells on CTL response
Périodique
Journal of Immunology
Auteur(s)
Fitch  F. W., Engers  H. D., Cerottini  J. C., Bruner  K. T.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
03/1976
Volume
116
Numéro
3
Pages
716-23
Notes
In Vitro
Journal Article
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
Irradiated cells obtained from MLC at the peak of the CTL response caused profound suppression of generation of CTL when added in small numbers at the initiation of primary MLC prepared with normal spleen cells. The inhibitory activity of the MLC cells was not affected by irradiation (1000 rads) but was abolished by treatment with anti-theta serum and complement. The suppression was immunologically specific. The response of A (H-2a) spleen cells toward C3H (H-2k) alloantigens was suppressed by irradiated MLC cells obtained from MLC prepared with A spleen cells and irradiated C3H-stimulating cells, whereas the response of A spleen cells toward DBA/2 (H-2d) alloantigens was affected relatively little. However, if irradiated C3H X DBA/2 F1 hybrid spleen cells were used to stimulate A spleen cells in MLC, addition of irradiated MLC cells having cytotoxic activity toward C3H antigens abolished the response to both C3H and DBA/2 antigens. The response to DBA/2 antigens was much less affected when a mixture of irradiated C3H and DBA/2 spleen cells was used as stimulating cells. Thus, the presence of MLC cells having cytotoxic activity toward one alloantigen abolished the response to another non-cross reacting antigen only when both antigens were present on the same F1 hybrid-stimulating cells. This suppression of generation of CTL by irradiated MLC cells apparently involves inactivation of alloantigen-bearing stimulating cells as a result of residual cytotoxic activity of the irradiated MLC cells. This mechanism may be active during the decline in CTL activity noted in the normal immune response in vivo and in vitro.
Mots-clé
Animals Antilymphocyte Serum/pharmacology Complement System Proteins Cytotoxicity Tests, Immunologic Dose-Response Relationship, Radiation Epitopes Immunity, Cellular/*radiation effects *Immunosuppression Lymphocyte Culture Test, Mixed Mice Mice, Inbred A Mice, Inbred C3H Mice, Inbred C57BL Mice, Inbred DBA Radiation Effects T-Lymphocytes/*cytology Time Factors X-Rays
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
03/03/2018 19:24
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