Crescent and star shapes of members of the Chlamydiales order: impact of fixative methods.

Détails

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Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_9045C2B71DA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Crescent and star shapes of members of the Chlamydiales order: impact of fixative methods.
Périodique
Antonie Van Leeuwenhoek
Auteur(s)
Rusconi B., Lienard J., Aeby S., Croxatto A., Bertelli C., Greub G.
ISSN
1572-9699 (Electronic)
ISSN-L
0003-6072
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
104
Numéro
4
Pages
521-532
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
Members of the Chlamydiales order all share a biphasic lifecycle alternating between small infectious particles, the elementary bodies (EBs) and larger intracellular forms able to replicate, the reticulate bodies. Whereas the classical Chlamydia usually harbours round-shaped EBs, some members of the Chlamydia-related families display crescent and star-shaped morphologies by electron microscopy. To determine the impact of fixative methods on the shape of the bacterial cells, different buffer and fixative combinations were tested on purified EBs of Criblamydia sequanensis, Estrella lausannensis, Parachlamydia acanthamoebae, and Waddlia chondrophila. A linear discriminant analysis was performed on particle metrics extracted from electron microscopy images to recognize crescent, round, star and intermediary forms. Depending on the buffer and fixatives used, a mixture of alternative shapes were observed in varying proportions with stars and crescents being more frequent in C. sequanensis and P. acanthamoebae, respectively. No tested buffer and chemical fixative preserved ideally the round shape of a majority of bacteria and other methods such as deep-freezing and cryofixation should be applied. Although crescent and star shapes could represent a fixation artifact, they certainly point towards a diverse composition and organization of membrane proteins or intracellular structures rather than being a distinct developmental stage.
Pubmed
Web of science
Création de la notice
24/10/2013 17:51
Dernière modification de la notice
24/03/2019 13:34
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