Synthesis of high-molecular-weight poly([R]-(-)-3-hydroxybutyrate) in transgenic Arabidopsis thaliana plant cells.

Détails

ID Serval
serval:BIB_90434AF988A5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Synthesis of high-molecular-weight poly([R]-(-)-3-hydroxybutyrate) in transgenic Arabidopsis thaliana plant cells.
Périodique
International Journal of Biological Macromolecules
Auteur(s)
Poirier Y., Somerville C., Schechtman L.A., Satkowski M.M., Noda I.
ISSN
0141-8130 (Print)
ISSN-L
0141-8130
Statut éditorial
Publié
Date de publication
1995
Volume
17
Numéro
1
Pages
7-12
Langue
anglais
Résumé
High-molecular-weight poly([R]-(-)-3-hydroxybutyrate) (PHB), a biodegradable thermoplastic, was produced from a suspension culture of transgenic Arabidopsis thaliana plant cells expressing two genes from the bacterium Alcaligenes eutrophus involved in the synthesis of PHB. The molecular structure of the plant-produced polymer was analysed by gas chromatography, mass spectrometry, proton nuclear magnetic resonance spectroscopy, infra-red spectroscopy, spectropolarimetry, differential scanning calorimetry, X-ray diffraction and size exclusion chromatography. The results indicate that the polymer from transgenic plants appears to have a chemical structure identical to that of PHB produced by bacteria. However, the molecular weight distribution of the plant-produced PHB was much broader than that of typical bacterial PHB.
Mots-clé
Alcaligenes/genetics, Arabidopsis/genetics, Arabidopsis/metabolism, Gas Chromatography-Mass Spectrometry, Genes, Bacterial, Hydroxybutyrates/chemistry, Hydroxybutyrates/metabolism, Macromolecular Substances, Magnetic Resonance Spectroscopy, Molecular Structure, Molecular Weight, Plants, Genetically Modified, Polyesters/chemistry, Polyesters/metabolism, X-Ray Diffraction
Pubmed
Web of science
Création de la notice
24/01/2008 20:42
Dernière modification de la notice
03/03/2018 19:24
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