MALDI-TOF MS-based drug susceptibility testing of pathogens: The example of Candida albicans and fluconazole.

Détails

ID Serval
serval:BIB_903E0C449ED3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
MALDI-TOF MS-based drug susceptibility testing of pathogens: The example of Candida albicans and fluconazole.
Périodique
Proteomics
Auteur(s)
Marinach C., Alanio A., Palous M., Kwasek S., Fekkar A., Brossas J.Y., Brun S., Snounou G., Hennequin C., Sanglard D., Datry A., Golmard J.L., Mazier D.
ISSN
1615-9861[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
9
Numéro
20
Pages
4627-4631
Langue
anglais
Résumé
MALDI-TOF MS can be used for the identification of microorganism species. We have extended its application to a novel assay of Candida albicans susceptibility to fluconazole, based on monitoring modifications of the proteome of yeast cells grown in the presence of varying drug concentrations. The method was accurate, and reliable, and showed full agreement with the Clinical Laboratory Standards Institute's reference method. This proof-of-concept demonstration highlights the potential for this approach to test other pathogens.
Mots-clé
Algorithm, Candida albicans, MALDI-TOF MS, Microbiology, Proteome profiling, Target for drugs, Mass-Spectrometry, Interpretive Breakpoints, European Committee, National Committee, Clsi M27-A2, Identification, Itraconazole, Infections, Spp.
Pubmed
Web of science
Création de la notice
24/11/2009 16:39
Dernière modification de la notice
03/03/2018 19:24
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