Alpha-tocopherol as a modulator of smooth muscle cell proliferation

Détails

ID Serval
serval:BIB_90312C517243
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Alpha-tocopherol as a modulator of smooth muscle cell proliferation
Périodique
Prostaglandins Leukotrienes and Essential Fatty Acids
Auteur(s)
Azzi  A., Boscoboinik  D., Clement  S., Marilley  D., Ozer  N. K., Ricciarelli  R., Tasinato  A.
ISSN
0952-3278 (Print)
Statut éditorial
Publié
Date de publication
10/1997
Volume
57
Numéro
4-5
Pages
507-14
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
The effects of alpha-tocopherol and beta-tocopherol have been studied in rat and human aortic smooth muscle cells. Alpha-tocopherol, but not beta-tocopherol, inhibited smooth muscle cell proliferation and protein kinase C in a dose-dependent manner, at concentrations ranging from 10 to 50 microM. Beta-tocopherol added simultaneously with alpha-tocopherol prevented both proliferation and protein kinase C inhibition. Protein kinase C inhibition was cell cycle-dependent and it was prevented by okadaic acid, a protein phosphatase inhibitor. Protein kinase C activity measured from aortas of cholesterol-fed rabbits was also inhibited by alpha-tocopherol. By using protein kinase C (PKC) isoform-specific inhibitors and immunoprecipitation reactions it was found that PKC-alpha was selectively inhibited by alpha-tocopherol. Further, an activation of protein phosphatase 2A by alpha-tocopherol was found, which caused PKC-alpha dephosphorylation and inhibition. Ultimately, this cascade of events at the level of cell signal transduction leads to the inhibition of smooth muscle cell proliferation.
Mots-clé
Animals Binding Sites Cell Cycle/drug effects Cell Division/*drug effects Humans Isoenzymes/antagonists & inhibitors/drug effects/metabolism Male Muscle, Smooth, Vascular/cytology/*drug effects Phosphoprotein Phosphatase/drug effects/metabolism Protein Kinase C/antagonists & inhibitors/drug effects/metabolism Protein Kinase C-alpha Rabbits Rats Vitamin E/*pharmacology
Pubmed
Web of science
Création de la notice
29/01/2008 9:44
Dernière modification de la notice
03/03/2018 19:24
Données d'usage