Cornea graft endothelial cells undergo apoptosis by way of an alternate (caspase-independent) pathway.

Details

Serval ID
serval:BIB_901AEF44920A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cornea graft endothelial cells undergo apoptosis by way of an alternate (caspase-independent) pathway.
Journal
Transplantation
Author(s)
Bourges J.L., Valamanesh F., Torriglia A., Jeanny J.C., Savoldelli M., Renard G., BenEzra D., de Kozak Y., Behar-Cohen F.
ISSN
0041-1337 (Print)
ISSN-L
0041-1337
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
78
Number
3
Pages
316-323
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish
Abstract
PURPOSE: To look for apoptosis pathways involved in corneal endothelial cell death during acute graft rejection and to evaluate the potential role of nitric oxide in this process.
MATERIALS AND METHODS: Corneal buttons from Brown-Norway rats were transplanted into Lewis rat corneas. At different time intervals after transplantation, apoptosis was assessed by diamino-2-phenylindol staining and annexin-V binding on flat-mount corneas, and by terminal transferase dUTP nick end labeling (TUNEL), caspase-3 dependent and leukocyte elastase inhibitor (LEI)/LDNase II caspase-independent pathways on sections. Inducible nitric oxide synthase (NOS-II) expression and the presence of nitrotyrosine were assayed by immunohistochemistry.
RESULTS: Graft endothelial cells demonstrated nuclear fragmentation and LEI nuclear translocation, annexin-V binding, and membranes bleb formation. Apoptosis associated with caspase-3 activity or TUNEL-positive reaction was not observed at any time either in the graft or in the recipient corneal endothelial cells. During 14 days posttransplantation, the recipient corneal endothelial cells remained unaltered and their number unchanged in all studied corneas. NOS-II was expressed in infiltrating cells present within the graft. This expression was closely associated with the presence of nitrotyrosine in endothelial and infiltrating cells.
CONCLUSION: During the time course of corneal graft rejection, graft endothelial cells undergo apoptosis. Apoptosis is caspase 3 independent and TUNEL negative and is, probably, carried out by an alternative pathway driven by an LEI/L-Dnase II. Peroxynitrite formation may be an additional mechanism for cell toxicity and programmed cell death of the graft endothelial cells during the rejection process in this model.
Keywords
Animals, Apoptosis/physiology, Caspases/metabolism, Corneal Transplantation/physiology, Endothelium, Vascular/cytology, Endothelium, Vascular/physiology, Rats, Rats, Inbred BN, Rats, Inbred Lew, Transplantation, Homologous
Pubmed
Web of science
Create date
19/11/2013 11:25
Last modification date
20/08/2019 14:53
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