Essential role of phosphoinositide metabolism in synaptic vesicle recycling.

Détails

ID Serval
serval:BIB_8FB0BB681E89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Essential role of phosphoinositide metabolism in synaptic vesicle recycling.
Périodique
Cell
Auteur(s)
Cremona O., Di Paolo G., Wenk M.R., Lüthi A., Kim W.T., Takei K., Daniell L., Nemoto Y., Shears S.B., Flavell R.A., McCormick D.A., De Camilli P.
ISSN
0092-8674
Statut éditorial
Publié
Date de publication
1999
Peer-reviewed
Oui
Volume
99
Numéro
2
Pages
179-188
Langue
anglais
Résumé
Growing evidence suggests that phosphoinositides play an important role in membrane traffic. A polyphosphoinositide phosphatase, synaptojanin 1, was identified as a major presynaptic protein associated with endocytic coated intermediates. We report here that synaptojanin 1-deficient mice exhibit neurological defects and die shortly after birth. In neurons of mutant animals, PI(4,5)P2 levels are increased, and clathrin-coated vesicles accumulate in the cytomatrix-rich area that surrounds the synaptic vesicle cluster in nerve endings. In cell-free assays, reduced phosphoinositide phosphatase activity correlated with increased association of clathrin coats with liposomes. Intracellular recording in hippocampal slices revealed enhanced synaptic depression during prolonged high-frequency stimulation followed by delayed recovery. These results provide genetic evidence for a crucial role of phosphoinositide metabolism in synaptic vesicle recycling.
Mots-clé
Animals, Cell-Free System, Cerebral Cortex, Coated Pits, Cell-Membrane, Endocytosis, Enzyme Inhibitors, Exons, Hippocampus, Membrane Potentials, Mice, Mice, Knockout, Microscopy, Electron, Nerve Endings, Nerve Tissue Proteins, Neurons, Phosphatidylinositols, Phosphoric Monoester Hydrolases, Synaptic Vesicles
Pubmed
Web of science
Open Access
Oui
Création de la notice
26/02/2009 15:45
Dernière modification de la notice
08/05/2019 21:56
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