Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.

Détails

ID Serval
serval:BIB_8F71152DFF48
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Control of cell division in Streptococcus pneumoniae by the conserved Ser/Thr protein kinase StkP.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Beilharz K., Nováková L., Fadda D., Branny P., Massidda O., Veening J.W.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2012
Volume
109
Numéro
15
Pages
E905-E913
Langue
anglais
Résumé
How the human pathogen Streptococcus pneumoniae coordinates cell-wall synthesis during growth and division to achieve its characteristic oval shape is poorly understood. The conserved eukaryotic-type Ser/Thr kinase of S. pneumoniae, StkP, previously was reported to phosphorylate the cell-division protein DivIVA. Consistent with a role in cell division, GFP-StkP and its cognate phosphatase, GFP-PhpP, both localize to the division site. StkP localization depends on its penicillin-binding protein and Ser/Thr-associated domains that likely sense uncross-linked peptidoglycan, because StkP and PhpP delocalize in the presence of antibiotics that target the latest stages of cell-wall biosynthesis and in cells that have stopped dividing. Time-lapse microscopy shows that StkP displays an intermediate timing of recruitment to midcell: StkP arrives shortly after FtsA but before DivIVA. Furthermore, StkP remains at midcell longer than FtsA, until division is complete. Cells mutated for stkP are perturbed in cell-wall synthesis and display elongated morphologies with multiple, often unconstricted, FtsA and DivIVA rings. The data show that StkP plays an important role in regulating cell-wall synthesis and controls correct septum progression and closure. Overall, our results indicate that StkP signals information about the cell-wall status to key cell-division proteins and in this way acts as a regulator of cell division.
Mots-clé
Anti-Bacterial Agents/pharmacology, Bacterial Proteins/chemistry, Bacterial Proteins/metabolism, Cell Division/drug effects, Cell Proliferation/drug effects, Cell Wall/drug effects, Cell Wall/metabolism, Conserved Sequence, Enzyme Activation/drug effects, Extracellular Space/drug effects, Extracellular Space/metabolism, Green Fluorescent Proteins/metabolism, Humans, Ligands, Models, Biological, Phosphorylation/drug effects, Protein Structure, Tertiary, Protein Transport/drug effects, Protein-Serine-Threonine Kinases/chemistry, Protein-Serine-Threonine Kinases/metabolism, Recombinant Fusion Proteins/metabolism, Streptococcus pneumoniae/cytology, Streptococcus pneumoniae/drug effects, Time Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
11/10/2016 16:30
Dernière modification de la notice
08/05/2019 21:55
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