An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.

Détails

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Etat: Serval
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_8F442D30F14F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile.
Périodique
PloS one
Auteur(s)
Pinto A.R., Paolicelli R., Salimova E., Gospocic J., Slonimsky E., Bilbao-Cortes D., Godwin J.W., Rosenthal N.A.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
7
Numéro
5
Pages
e36814
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Cardiac tissue macrophages (cTMs) are a previously uncharacterised cell type that we have identified and characterise here as an abundant GFP(+) population within the adult Cx(3)cr1(GFP/+) knock-in mouse heart. They comprise the predominant myeloid cell population in the myocardium, and are found throughout myocardial interstitial spaces interacting directly with capillary endothelial cells and cardiomyocytes. Flow cytometry-based immunophenotyping shows that cTMs exhibit canonical macrophage markers. Gene expression analysis shows that cTMs (CD45(+)CD11b(+)GFP(+)) are distinct from mononuclear CD45(+)CD11b(+)GFP(+) cells sorted from the spleen and brain of adult Cx(3)cr1(GFP/+) mice. Gene expression profiling reveals that cTMs closely resemble alternatively-activated anti-inflammatory M2 macrophages, expressing a number of M2 markers, including Mrc1, CD163, and Lyve-1. While cTMs perform normal tissue macrophage homeostatic functions, they also exhibit a distinct phenotype, involving secretion of salutary factors (including IGF-1) and immune modulation. In summary, the characterisation of cTMs at the cellular and molecular level defines a potentially important role for these cells in cardiac homeostasis.
Mots-clé
Animals, Antigens, CD/biosynthesis, Antigens, Differentiation/biosynthesis, Antigens, Differentiation, Myelomonocytic/biosynthesis, Brain/cytology, Brain/metabolism, CD11b Antigen/biosynthesis, Endothelial Cells/cytology, Endothelial Cells/metabolism, Glycoproteins/biosynthesis, Homeostasis/physiology, Insulin-Like Growth Factor I/biosynthesis, Leukocyte Common Antigens/biosynthesis, Macrophage Activation/physiology, Macrophages/cytology, Macrophages/metabolism, Mice, Mice, Transgenic, Myocardium/cytology, Myocardium/metabolism, Myocytes, Cardiac/cytology, Myocytes, Cardiac/metabolism, Receptors, Cell Surface/biosynthesis, Spleen/cytology, Spleen/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/12/2018 11:44
Dernière modification de la notice
18/06/2019 6:26
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