Transformed and nontransformed human T lymphocytes migrate to skin in a chimeric human skin/SCID mouse model.

Détails

ID Serval
serval:BIB_8F1463E4A1BE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transformed and nontransformed human T lymphocytes migrate to skin in a chimeric human skin/SCID mouse model.
Périodique
Journal of Investigative Dermatology
Auteur(s)
Rosenblatt-Velin N., Arrighi J.F., Dietrich P.Y., Schnuriger V., Masouyé I., Hauser C.
ISSN
0022-202X
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
109
Numéro
6
Pages
744-750
Langue
anglais
Résumé
To study human T cell migration to human skin in vivo, we grafted severe combined immunodeficient mice with 500-microm thick human skin. Two weeks after grafting, epidermal and dermal structures in the grafts were of human origin. When we intraperitoneally injected grafted mice with clones of the human HUT-78 T cell line derived from a patient with cutaneous T cell lymphoma and Sézary syndrome, we detected in the grafts the rare Vbeta23-Jbeta1.2 T cell receptor transcripts characteristic for the HUT-78 clones. These signals were found 2-6 d after cell injection in about 40% of the grafted and HUT-78 cell injected mice but not in grafts from mice that received no exogenous T cells. In contrast to HUT-78 cells, which only accumulate in low number, grafts topically challenged with nickel sufate in vaseline from mice that were injected with autologous nickel-reactive T cell lines led to massive accumulation of T cells within 3 d. Only scattered T cells accumulated in the skin when grafted mice received vaseline plus T cells, nickel sulfate alone, T cells alone, or nickel sulfate plus an allogeneic nickel-nonreactive T cell clone. When the T cell lines were labeled with the fluorochrome PKH-26 before cell injection, spots of fluorescent label in the size and shape of cells were found in the grafts challenged with nickel. Together, these results clearly demonstrate that human T cells can migrate to human skin in this chimeric human/mouse model.
Mots-clé
Animals, Cell Movement, Chimera, Humans, Lymphocyte Activation, Mice, Mice, SCID, Nickel/pharmacology, RNA, Messenger/analysis, Receptors, Antigen, T-Cell, alpha-beta/genetics, Skin/immunology, Skin Transplantation, T-Lymphocytes/physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
16/10/2009 9:55
Dernière modification de la notice
08/05/2019 21:54
Données d'usage