Two specific populations of GABAergic neurons originating from the medial and the caudal ganglionic eminences aid in proper navigation of callosal axons.

Détails

Ressource 1Télécharger: BIB_8ECDB337A3F3.P001.pdf (3185.75 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_8ECDB337A3F3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Two specific populations of GABAergic neurons originating from the medial and the caudal ganglionic eminences aid in proper navigation of callosal axons.
Périodique
Developmental Neurobiology
Auteur(s)
Niquille M., Minocha S., Hornung J.P., Rufer N., Valloton D., Kessaris N., Alfonsi F., Vitalis T., Yanagawa Y., Devenoges C., Dayer A., Lebrand C.
ISSN
1932-846X (Electronic)
ISSN-L
1932-8451
Statut éditorial
Publié
Date de publication
2013
Volume
73
Numéro
9
Pages
647-672
Langue
anglais
Résumé
The corpus callosum (CC) plays a crucial role in interhemispheric communication. It has been shown that CC formation relies on the guidepost cells located in the midline region that include glutamatergic and GABAergic neurons as well as glial cells. However, the origin of these guidepost GABAergic neurons and their precise function in callosal axon pathfinding remain to be investigated. Here, we show that two distinct GABAergic neuronal subpopulations converge toward the midline prior to the arrival of callosal axons. Using in vivo and ex vivo fate mapping we show that CC GABAergic neurons originate in the caudal and medial ganglionic eminences (CGE and MGE) but not in the lateral ganglionic eminence (LGE). Time lapse imaging on organotypic slices and in vivo analyses further revealed that CC GABAergic neurons contribute to the normal navigation of callosal axons. The use of Nkx2.1 knockout (KO) mice confirmed a role of these neurons in the maintenance of proper behavior of callosal axons while growing through the CC. Indeed, using in vitro transplantation assays, we demonstrated that both MGE- and CGE-derived GABAergic neurons exert an attractive activity on callosal axons. Furthermore, by combining a sensitive RT-PCR technique with in situ hybridization, we demonstrate that CC neurons express multiple short and long range guidance cues. This study strongly suggests that MGE- and CGE-derived interneurons may guide CC axons by multiple guidance mechanisms and signaling pathways. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 647-672, 2013.
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/09/2013 10:16
Dernière modification de la notice
08/05/2019 21:53
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