Characterization of a trimeric complex containing Oct-1, SNAPc, and DNA.

Détails

ID Serval
serval:BIB_8EC78F4494AB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of a trimeric complex containing Oct-1, SNAPc, and DNA.
Périodique
Journal of Biological Chemistry
Auteur(s)
Ford E., Hernandez N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
06/1997
Volume
272
Numéro
25
Pages
16048-16055
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The human small nuclear (sn) RNA promoters contain a proximal sequence element (PSE), which recruits the basal transcription factor SNAPc, and a distal sequence element characterized by an octamer sequence, which recruits the POU domain transcription factor Oct-1. The Oct-1 POU domain and SNAPc bind cooperatively to probes containing a PSE and an octamer sequence, and this effect contributes to efficient transcription in vitro. In vivo, however, Oct-1 regions outside of the POU domain can activate snRNA gene transcription. Here, we have examined whether the role of these regions is to contribute to cooperative binding with SNAPc. We find that they indeed improve cooperative binding, but most of the effect is nevertheless mediated by just the POU domain. This suggests that Oct-1 activates transcription of snRNA genes in at least two steps, recruitment of SNAPc mediated primarily by the POU domain, and a later step mediated by regions outside of the POU domain. We also show that a PSE-binding complex observed in nuclear extracts consists of Oct-1 and SNAPc. Although Oct-1 cannot bind effectively to the PSE probe on its own, in the complex it contacts DNA. Thus, in a nuclear extract, SNAPc can recruit Oct-1 to a probe to which Oct-1 cannot bind on its own.
Mots-clé
Base Sequence, Binding Sites, DNA/metabolism, DNA-Binding Proteins/metabolism, Electrophoresis, Polyacrylamide Gel, Host Cell Factor C1, Humans, Macromolecular Substances, Molecular Sequence Data, Octamer Transcription Factor-1, Octamer Transcription Factor-2, Proteins, Transcription Factors/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 17:34
Dernière modification de la notice
08/05/2019 21:53
Données d'usage