Transcription factor CTF1 acts as a chromatin domain boundary that shields human telomeric genes from silencing.

Détails

ID Serval
serval:BIB_8EBBCF62537B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcription factor CTF1 acts as a chromatin domain boundary that shields human telomeric genes from silencing.
Périodique
Molecular and Cellular Biology
Auteur(s)
Esnault G., Majocchi S., Martinet D., Besuchet-Schmutz N., Beckmann J.S., Mermod N.
ISSN
1098-5549[electronic], 0270-7306[linking]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
29
Numéro
9
Pages
2409-2418
Langue
anglais
Résumé
Telomeres are associated with chromatin-mediated silencing of genes in their vicinity. However, how epigenetic markers mediate mammalian telomeric silencing and whether specific proteins may counteract this effect are not known. We evaluated the ability of CTF1, a DNA- and histone-binding transcription factor, to prevent transgene silencing at human telomeres. CTF1 was found to protect a gene from silencing when its DNA-binding sites were interposed between the gene and the telomeric extremity, while it did not affect a gene adjacent to the telomere. Protein fusions containing the CTF1 histone-binding domain displayed similar activities, while mutants impaired in their ability to interact with the histone did not. Chromatin immunoprecipitation indicated the propagation of a hypoacetylated histone structure to various extents depending on the telomere. The CTF1 fusion protein was found to recruit the H2A.Z histone variant at the telomeric locus and to restore high histone acetylation levels to the insulated telomeric transgene. Histone lysine trimethylations were also increased on the insulated transgene, indicating that these modifications may mediate expression rather than silencing at human telomeres. Overall, these results indicate that transcription factors can act to delimit chromatin domain boundaries at mammalian telomeres, thereby blocking the propagation of a silent chromatin structure.
Mots-clé
Chromatin/metabolism, DNA Methylation, Gene Silencing, Genes, Reporter, Hela Cells, Histones/metabolism, Humans, In Situ Hybridization, Fluorescence, NFI Transcription Factors/genetics, NFI Transcription Factors/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Telomere/genetics, Telomere/metabolism, Transgenes
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/03/2009 12:11
Dernière modification de la notice
20/08/2019 14:52
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