Membrane contact sites between apicoplast and ER in Toxoplasma gondii revealed by electron tomography.

Détails

ID Serval
serval:BIB_8EB4D138B251
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Membrane contact sites between apicoplast and ER in Toxoplasma gondii revealed by electron tomography.
Périodique
Traffic
Auteur(s)
Tomova C., Humbel B.M., Geerts W.J.C., Entzeroth R., Holthuis J.C., Verkleij A.J.
ISSN
1600-0854 (Electronic)
ISSN-L
1398-9219
Statut éditorial
Publié
Date de publication
2009
Volume
10
Numéro
10
Pages
1471-1480
Langue
anglais
Résumé
Toxoplasma gondii is an obligate intracellular parasite from the phylum Apicomplexa. A hallmark of these protozoans is the presence of a unique apical complex of organelles that includes the apicoplast, a plastid acquired by secondary endosymbiosis. The apicoplast is indispensible for parasite viability. It harbours a fatty acid biosynthesis type II (FAS II) pathway and plays a key role in the parasite lipid metabolism. Possibly, the apicoplast provides components for the establishment and the maturation of the parasitophorous vacuole, ensuring the successful infection of the host cell. This implies the presence of a transport mechanism for fast and accurate allocation of lipids between the apicoplast and other membrane-bound compartments in the parasite cell. Using a combination of high-pressure freezing, freeze-substitution and electron tomography, we analysed the ultrastructural organization of the apicoplast of T. gondii in relation with the endoplasmic reticulum (ER). This allowed us to clearly show the presence of four continuous membranes surrounding the apicoplast. We present, for the first time, the existence of membrane contact sites between the apicoplast outermost membrane and the ER. We describe the morphological characteristics of these structures and discuss their potential significance for the subcellular distribution of lipids in the parasite.
Mots-clé
Animals, Biological Transport, Cercopithecus aethiops, Cryoultramicrotomy, Electron Microscope Tomography, Endoplasmic Reticulum/metabolism, Endoplasmic Reticulum/parasitology, Fatty Acid Synthetase Complex, Type II/biosynthesis, Fatty Acids/biosynthesis, Host-Parasite Interactions, Intracellular Membranes/metabolism, Intracellular Membranes/ultrastructure, Lipid Metabolism, Models, Biological, Organelles/metabolism, Organelles/ultrastructure, Symbiosis/physiology, Toxoplasma/enzymology, Toxoplasma/metabolism, Vacuoles/metabolism, Vacuoles/parasitology, Vero Cells
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/02/2012 19:38
Dernière modification de la notice
08/05/2019 21:53
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