Article: article from journal or magazin.
Coordinate control of Na,K-atpase mRNA expression by aldosterone, vasopressin and cell sodium delivery in the cortical collecting duct
Cellular and Molecular Biololgy
Journal Article --- Old month value: Mar
We have examined the respective influence of aldosterone, vasopressin and cell sodium delivery on Na+,K+-ATPase expression. The level of expression of the mRNA encoding for the alpha1- and beta1-subunits of Na+,K+-ATPase was evaluated in cortical collecting duct (CCD) cells from rats under different aldosterone status, in cells from the rat CCD cell line RCCD1 treated or not with vasopressin and in CCD cells from mice inactivated or not for the a-subunit of the epithelial sodium channel. The amount of mRNA was determined by in situ hybridization. Both aldosterone and vasopressin up-regulate transcripts encoding for the alpha1-subunit of Na+,K+-ATPase while beta1 is unaltered. Interestingly, when cell sodium entry was largely reduced (alphaENaC knock-out mice), the amount of transcripts encoding for the alpha1-subunit of Na+,K+-ATPase was significantly decreased in spite of high plasma aldosterone concentrations. No effect was observed on beta1-subunit. Altogether, these results suggest a coordinated hormonal and ionic control of Na+,K+-ATPase expression by different transcriptional pathways (steroid-receptor, cAMP-dependent and Na+dependent) in CCD cells. These regulations affect only alpha1-subunit of Na,K+-ATPase but not beta1.
Adrenalectomy Aldosterone/*pharmacology Animals Cell Line Epithelial Sodium Channel In Situ Hybridization Kidney Cortex/drug effects/enzymology Kidney Tubules, Collecting/drug effects/*enzymology/physiology Male Mice Mice, Knockout Na(+)-K(+)-Exchanging ATPase/genetics/*metabolism Protein Subunits RNA, Messenger/metabolism Rats Rats, Sprague-Dawley Renal Agents/pharmacology Sodium/*metabolism Sodium Channels/genetics/metabolism Vasopressins/*pharmacology
Web of science
Last modification date