Airway microbiota signals anabolic and catabolic remodeling in the transplanted lung.
Details
State: Public
Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_8E8414112FB7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Airway microbiota signals anabolic and catabolic remodeling in the transplanted lung.
Journal
The Journal of allergy and clinical immunology
Working group(s)
SysCLAD Consortium
Contributor(s)
Jougon J., Velly J.F., Rozé H., Blanchard E., Dromer C., Antoine M., Cappello M., Ruiz M., Sokolow Y., Vanden Eynden F., Van Nooten G., Barvais L., Berré J., Brimioulle S., De Backer D., Créteur J., Engelman E., Huybrechts I., Ickx B., Preiser TJC, Tuna T., Van Obberghe L., Vancutsem N., Vincent J.L., De Vuyst P., Etienne I., Féry F., Jacobs F., Knoop C., Vachiéry J.L., Van den Borne P., Wellemans I., Amand G., Collignon L., Giroux M., Angelescu D., Chavanon O., Hacini R., Pirvu A., Porcu P., Albaladejo P., Allègre C., Bataillard A., Bedague D., Briot E., Casez-Brasseur M., Colas D., Dessertaine G., Durand M., Francony G., Hebrard A., Marino M.R., Oummahan B., Protar D., Rehm D., Robin S., Rossi-Blancher M., Augier C., Bedouch P., Boignard A., Bouvaist H., Briault A., Camara B., Claustre J., Chanoine S., Dubuc M., Quétant S., Maurizi J., Pavèse P., Pison C., Saint-Raymond C., Wion N., Chérion C., Grima R., Jegaden O., Maury J.M., Tronc F., Flamens C., Paulus S., Mornex J.F., Philit F., Senechal A., Glérant J.C., Turquier S., Gamondes D., Chalabresse L., Thivolet-Bejui F., Barnel C., Dubois C., Tiberghien A., Le Pimpec-Barthes F., Bel A., Mordant P., Achouh P., Boussaud V., Guillemain R., Méléard D., Bricourt M.O., Cholley B., Pezella V., Brioude G., D'Journo X.B., Doddoli C., Thomas P., Trousse D., Dizier S., Leone M., Papazian L., Bregeon F., Basire A., Coltey B., Dufeu N., Dutau H., Garcia S., Gaubert J.Y., Gomez C., Laroumagne S., Nieves A., Picard L.C., Reynaud-Gaubert M., Secq V., Mouton G., Baron O., Lacoste P., Perigaud C., Roussel J.C., Danner I., Haloun A., Magnan A., Tissot A., Lepoivre T., Treilhaud M., Botturi-Cavaillès K., Brouard S., Danger R., Loy J., Morisset M., Pain M., Pares S., Reboulleau D., Royer P.J., Fabre D., Fadel E., Mercier O., Mussot S., Stephan F., Viard P., Cerrina J., Dorfmuller P., Ghigna S.M., Hervén P., Le Roy Ladurie F., Le Pavec J., Thomas de Montpreville V., Lamrani L., Castier Y., Mordant P., Cerceau P., Augustin P., Jean-Baptiste S., Boudinet S., Montravers P., Brugière O., Dauriat G., Jébrak G., Mal H., Marceau A., Métivier A.C., Thabut G., Lhuillier E., Dupin C., Bunel V., Falcoz P., Massard G., Santelmo N., Ajob G., Collange O., Helms O., Hentz J., Roche A., Bakouboula B., Degot T., Dory A., Hirschi S., Ohlmann-Caillard S., Kessler L., Kessler R., Schuller A., Bennedif K., Vargas S., Stauder J., Ali-Azouaou S., Bonnette P., Chapelier A., Puyo P., Sage E., Bresson J., Caille V., Cerf C., Devaquet J., Dumans-Nizard V., Felten M.L., Fischler M., Si Larbi A.G., Leguen M., Ley L., Liu N., Trebbia G., De Miranda S., Douvry B., Gonin F., Grenet D., Hamid A.M., Neveu H., Parquin F., Picard C., Roux A., Stern M., Bouillioud F., Cahen P., Colombat M., Dautricourt C., Delahousse M., D'Urso B., Gravisse J., Guth A., Hillaire S., Honderlick P., Lequintrec M., Longchampt E., Mellot F., Scherrer A., Temagoult L., Tricot L., Vasse M., Veyrie C., Zemoura L., Berjaud J., Brouchet L., Dahan M., Mathe F.O., Benahoua H., DaCosta M., Serres I., Merlet-Dupuy V., Grigoli M., Didier A., Murris M., Crognier L., Fourcade O., Jougon J., Velly J.F., Rozé H., Blanchard E., Dromer C., Antoine M., Cappello M., Ruiz M., Sokolow Y., Vanden Eynden F., Van Nooten G., Barvais L., Berré J., Brimioulle S., De Backer D., Créteur J., Engelman E., Huybrechts I., Ickx B., Preiser TJC, Tuna T., Van Obberghe L., Vancutsem N., Vincent J.L., De Vuyst P., Etienne I., Féry F., Jacobs F., Knoop C., Vachiéry J.L., Van den Borne P., Wellemans I., Amand G., Collignon L., Giroux M., Angelescu D., Chavanon O., Hacini R., Pirvu A., Porcu P., Albaladejo P., Allègre C., Bataillard A., Bedague D., Briot E., Casez-Brasseur M., Colas D., Dessertaine G., Durand M., Francony G., Hebrard A., Marino M.R., Oummahan B., Protar D., Rehm D., Robin S., Rossi-Blancher M., Augier C., Bedouch P., Boignard A., Bouvaist H., Briault A., Camara B., Claustre J., Chanoine S., Dubuc M., Quétant S., Maurizi J., Pavèse P., Pison C., Saint-Raymond C., Wion N., Chérion C., Grima R., Jegaden O., Maury J.M., Tronc F., Flamens C., Paulus S., Mornex J.F., Philit F., Senechal A., Glérant J.C., Turquier S., Gamondes D., Chalabresse L., Thivolet-Bejui F., Barnel C., Dubois C., Tiberghien A., Le Pimpec-Barthes F., Bel A., Mordant P., Achouh P., Boussaud V., Guillemain R., Méléard D., Bricourt M.O., Cholley B., Pezella V., Brioude G., D'Journo X.B., Doddoli C., Thomas P., Trousse D., Dizier S., Leone M., Papazian L., Bregeon F., Basire A., Coltey B., Dufeu N., Dutau H., Garcia S., Gaubert J.Y., Gomez C., Laroumagne S., Nieves A., Picard L.C., Reynaud-Gaubert M., Secq V., Mouton G., Baron O., Lacoste P., Perigaud C., Roussel J.C., Danner I., Haloun A., Magnan A., Tissot A., Lepoivre T., Treilhaud M., Botturi-Cavaillès K., Brouard S., Danger R., Loy J., Morisset M., Pain M., Pares S., Reboulleau D., Royer P.J., Fabre D., Fadel E., Mercier O., Mussot S., Stephan F., Viard P., Cerrina J., Dorfmuller P., Ghigna S.M., Hervén P., Le Roy Ladurie F., Le Pavec J., Thomas de Montpreville V., Lamrani L., Castier Y., Mordant P., Cerceau P., Augustin P., Jean-Baptiste S., Boudinet S., Montravers P., Brugière O., Dauriat G., Jébrak G., Mal H., Marceau A., Métivier A.C., Thabut G., Lhuillier E., Dupin C., Bunel V., Falcoz P., Massard G., Santelmo N., Ajob G., Collange O., Helms O., Hentz J., Roche A., Bakouboula B., Degot T., Dory A., Hirschi S., Ohlmann-Caillard S., Kessler L., Kessler R., Schuller A., Bennedif K., Vargas S., Stauder J., Ali-Azouaou S., Bonnette P., Chapelier A., Puyo P., Sage E., Bresson J., Caille V., Cerf C., Devaquet J., Dumans-Nizard V., Felten M.L., Fischler M., Si Larbi A.G., Leguen M., Ley L., Liu N., Trebbia G., De Miranda S., Douvry B., Gonin F., Grenet D., Hamid A.M., Neveu H., Parquin F., Picard C., Roux A., Stern M., Bouillioud F., Cahen P., Colombat M., Dautricourt C., Delahousse M., D'Urso B., Gravisse J., Guth A., Hillaire S., Honderlick P., Lequintrec M., Longchampt E., Mellot F., Scherrer A., Temagoult L., Tricot L., Vasse M., Veyrie C., Zemoura L., Berjaud J., Brouchet L., Dahan M., Mathe F.O., Benahoua H., DaCosta M., Serres I., Merlet-Dupuy V., Grigoli M., Didier A., Murris M., Crognier L., Fourcade O., Krueger T., Ris H.B., Gonzalez M., Jolliet P., Marcucci C., Chollet M., Gronchi F., Courbon C., Berutto C., Manuel O., Koutsokera A., Aubert J.D., Nicod L.P., Mouraux S., Bernasconi E., Pattaroni C., Marsland B.J., Soccal P.M., Rochat T., Lücker L.M., Hillinger S., Inci I., Weder W., Schuepbach R., Zalunardo M., Benden C., Schuurmans M.M., Gaspert A., Holzmann D., Müller N., Schmid C., Vrugt B., Fritz A., Maier D., Deplanche K., Koubi D., Ernst F., Paprotka T., Schmitt M., Wahl B., Boissel J.P., Olivera-Botello G., Trocmé C., Toussaint B., Bourgoin-Voillard S., Sève M., Benmerad M., Siroux V., Slama R., Auffray C., Charron D., Lefaudeux D., Pellet J.
ISSN
1097-6825 (Electronic)
ISSN-L
0091-6749
Publication state
Published
Issued date
02/2018
Peer-reviewed
Oui
Volume
141
Number
2
Pages
718-729.e7
Language
english
Notes
Publication types: Clinical Trial ; Journal Article ; Multicenter Study ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Homeostatic turnover of the extracellular matrix conditions the structure and function of the healthy lung. In lung transplantation, long-term management remains limited by chronic lung allograft dysfunction, an umbrella term used for a heterogeneous entity ultimately associated with pathological airway and/or parenchyma remodeling.
This study assessed whether the local cross-talk between the pulmonary microbiota and host cells is a key determinant in the control of lower airway remodeling posttransplantation.
Microbiota DNA and host total RNA were isolated from 189 bronchoalveolar lavages obtained from 116 patients post lung transplantation. Expression of a set of 11 genes encoding either matrix components or factors involved in matrix synthesis or degradation (anabolic and catabolic remodeling, respectively) was quantified by real-time quantitative PCR. Microbiota composition was characterized using 16S ribosomal RNA gene sequencing and culture.
We identified 4 host gene expression profiles, among which catabolic remodeling, associated with high expression of metallopeptidase-7, -9, and -12, diverged from anabolic remodeling linked to maximal thrombospondin and platelet-derived growth factor D expression. While catabolic remodeling aligned with a microbiota dominated by proinflammatory bacteria (eg, Staphylococcus, Pseudomonas, and Corynebacterium), anabolic remodeling was linked to typical members of the healthy steady state (eg, Prevotella, Streptococcus, and Veillonella). Mechanistic assays provided direct evidence that these bacteria can impact host macrophage-fibroblast activation and matrix deposition.
Host-microbes interplay potentially determines remodeling activities in the transplanted lung, highlighting new therapeutic opportunities to ultimately improve long-term lung transplant outcome.
This study assessed whether the local cross-talk between the pulmonary microbiota and host cells is a key determinant in the control of lower airway remodeling posttransplantation.
Microbiota DNA and host total RNA were isolated from 189 bronchoalveolar lavages obtained from 116 patients post lung transplantation. Expression of a set of 11 genes encoding either matrix components or factors involved in matrix synthesis or degradation (anabolic and catabolic remodeling, respectively) was quantified by real-time quantitative PCR. Microbiota composition was characterized using 16S ribosomal RNA gene sequencing and culture.
We identified 4 host gene expression profiles, among which catabolic remodeling, associated with high expression of metallopeptidase-7, -9, and -12, diverged from anabolic remodeling linked to maximal thrombospondin and platelet-derived growth factor D expression. While catabolic remodeling aligned with a microbiota dominated by proinflammatory bacteria (eg, Staphylococcus, Pseudomonas, and Corynebacterium), anabolic remodeling was linked to typical members of the healthy steady state (eg, Prevotella, Streptococcus, and Veillonella). Mechanistic assays provided direct evidence that these bacteria can impact host macrophage-fibroblast activation and matrix deposition.
Host-microbes interplay potentially determines remodeling activities in the transplanted lung, highlighting new therapeutic opportunities to ultimately improve long-term lung transplant outcome.
Keywords
Adult, Airway Remodeling/immunology, Bacteria/classification, Bacteria/immunology, Extracellular Matrix/immunology, Extracellular Matrix/pathology, Female, Fibroblasts/immunology, Fibroblasts/pathology, Humans, Lung/immunology, Lung/microbiology, Lung/pathology, Lung Transplantation, Macrophages/immunology, Macrophages/pathology, Male, Microbiota/immunology, Middle Aged, Signal Transduction/immunology, Airway remodeling, fibroblasts, macrophages, matrix, microbiota
Pubmed
Web of science
Open Access
Yes
Create date
28/08/2017 10:54
Last modification date
19/12/2023 7:23
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