Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.

Détails

ID Serval
serval:BIB_8E7118AB14A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Disuse deterioration of human skeletal muscle challenged by resistive exercise superimposed with vibration: evidence from structural and proteomic analysis.
Périodique
Faseb Journal : Official Publication of the Federation of American Societies For Experimental Biology
Auteur(s)
Salanova M., Gelfi C., Moriggi M., Vasso M., Viganò A., Minafra L., Bonifacio G., Schiffl G., Gutsmann M., Felsenberg D., Cerretelli P., Blottner D.
ISSN
1530-6860 (Electronic)
ISSN-L
0892-6638
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
28
Numéro
11
Pages
4748-4763
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
In the present bed rest (BR) study, 23 volunteers were randomized into 3 subgroups: 60 d BR control (Ctr); BR with resistive exercise (RE; lower-limb load); and resistive vibration exercise (RVE; RE with superimposed vibration). The aim was to analyze by confocal and electron microscopy the effects of vibration on myofibril and filament integrity in soleus (Sol) and vastus lateralis (VL) muscle; differential proteomics of contractile, cytoskeletal, and costameric proteins (TN-C, ROCK1, and FAK); and expression of PGC1α and atrophy-related master genes MuRF1 and MuRF2. RVE (but not RE) preserved myofiber size and phenotype in Sol and VL by overexpressing MYBPC1 (42%, P ≤ 0.01), WDR1 (39%, P ≤ 0.01), sarcosin (84%, P ≤ 0.01), and CKM (20%, P ≤ 0.01) and prevented myofibrillar ultrastructural damage as detectable by MuRF1 expression. In Sol, cytoskeletal and contractile proteins were normalized by RVE, and TN-C increased (59%, P ≤ 0.01); the latter also with RE (108%, P ≤ 0.01). In VL, the outcomes of both RVE (acting on sarcosin and desmin) and RE (by way of troponinT-slow and MYL2) were similar. RVE appears to be a highly efficient countermeasure protocol against muscle atrophy and ultrastructural and molecular dysregulation induced by chronic disuse.
Mots-clé
Adult, Bed Rest/methods, Exercise Therapy, Humans, Male, Middle Aged, Muscle Contraction/physiology, Muscle, Skeletal/metabolism, Muscle, Skeletal/pathology, Muscular Atrophy/metabolism, Muscular Atrophy/therapy, Proteomics, Vibration, Young Adult
Pubmed
Web of science
Création de la notice
18/10/2016 16:07
Dernière modification de la notice
20/08/2019 14:52
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