The protein Mago provides a link between splicing and mRNA localization.

Détails

ID Serval
serval:BIB_8E6DB955A1BC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The protein Mago provides a link between splicing and mRNA localization.
Périodique
EMBO Reports
Auteur(s)
Le Hir H., Gatfield D., Braun I.C., Forler D., Izaurralde E.
ISSN
1469-221X (Print)
ISSN-L
1469-221X
Statut éditorial
Publié
Date de publication
2001
Volume
2
Numéro
12
Pages
1119-1124
Langue
anglais
Résumé
The proteins Mago and Y14 are evolutionarily conserved binding partners. Y14 is a component of the exon-exon junction complex (EJC), deposited by the spliceosome upstream of messenger RNA (mRNA) exon-exon junctions. The EJC is implicated in post-splicing events such as mRNA nuclear export and nonsense-mediated mRNA decay. Drosophila Mago is essential for the localization of oskar mRNA to the posterior pole of the oocyte, but the functional role of Mago in other species is unknown. We show that Mago is a bona fide component of the EJC. Like Y14, Mago escorts spliced mRNAs to the cytoplasm, providing a direct functional link between splicing and the downstream process of mRNA localization. Mago/Y14 heterodimers are essential in cultured Drosophila cells. Taken together, these results suggest that, in addition to its specialized function in mRNA localization, Mago plays an essential role in other steps of mRNA metabolism.
Mots-clé
Animals, Biological Transport, Cell Division, Cell Line, Cell Nucleus Structures/metabolism, Cytoplasm/metabolism, DNA/genetics, Dimerization, Drosophila/cytology, Drosophila/genetics, Drosophila Proteins, Evolution, Molecular, Exons/genetics, HeLa Cells, Humans, Male, Nuclear Proteins/genetics, Nuclear Proteins/metabolism, Polymerase Chain Reaction, Protein Binding, RNA Splicing/genetics, RNA, Messenger/chemistry, RNA, Messenger/genetics, RNA-Binding Proteins/genetics, RNA-Binding Proteins/metabolism, Testis, Transfection, Xenopus
Pubmed
Web of science
Création de la notice
12/12/2012 12:22
Dernière modification de la notice
03/03/2018 19:18
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