Cryoconservation et transplantations osseuses [Cryopreservation and bone transplantation]

Détails

ID Serval
serval:BIB_8E5D0EB3EF72
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cryoconservation et transplantations osseuses [Cryopreservation and bone transplantation]
Périodique
Swiss Surgery
Auteur(s)
Dutoit M.
ISSN
1023-9332
Statut éditorial
Publié
Date de publication
1995
Numéro
4
Pages
202-204
Langue
français
Résumé
Massive bone allografts allow us to treat easily large osseous defects. This study reports our experience with 90 massive allografts of whom 50 had more than 2 years follow-up. The reconstruction was in half of cases the femur, in ten patients one or more vertebra, hemipelvis in five, humerus in four and tibia in three cases. The main indication was tumor surgery: 30 malignant, 6 benigns, but also for the treatment of massive bone loss in ten prothesis replacement and in 4 miscellaneous pathology (malformation, infection ...). The massive allografts allow us more anatomic reconstructions and particularly in young patients we can observe some rehabitation of the graft by host cells. The complication rate is quite high mainly mechanical (10 fractures) in the first two years following surgery, but also 2 sepsis and 2 sciatic palsy. Simultaneously an experimental and morphological study of cartilaginous fragments was undertaken: at first, we define the best method of fixation and examination of human articular cartilaginous fragments by electronic microscopy. This method was then applied to cartilaginous fragments liable to various cryoprotective solutions, stocked for various periods at different temperature and subjects to programmed cryocongelation. So we are able to define the best conditions of cryoconservation of human adult articular cartilage fragments and we can observe that 70-80% of cells maintain normal morphological structure suggesting a perfect cellular viability.
Mots-clé
Adult, Bone Neoplasms/pathology, Bone Neoplasms/surgery, Bone Transplantation/methods, Bone Transplantation/pathology, Cartilage, Articular/pathology, Cartilage, Articular/transplantation, Cryopreservation/methods, Female, Follow-Up Studies, Humans, Male, Postoperative Complications/pathology, Transplantation, Homologous, Treatment Outcome
Pubmed
Création de la notice
28/01/2008 13:14
Dernière modification de la notice
03/03/2018 19:18
Données d'usage