Differential sensitivity of GLUT1- and GLUT2-expressing beta cells to streptozotocin.

Details

Serval ID
serval:BIB_8E5B861B6A33
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Differential sensitivity of GLUT1- and GLUT2-expressing beta cells to streptozotocin.
Journal
Biochemical and Biophysical Research Communications
Author(s)
Hosokawa M., Dolci W., Thorens B.
ISSN
0006-291X[print], 0006-291X[linking]
Publication state
Published
Issued date
12/2001
Volume
289
Number
5
Pages
1114-1117
Language
english
Notes
Publication types: Comparative Study ; In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Streptozotocin injection in animals destroys pancreatic beta cells, leading to insulinopenic diabetes. Here, we evaluated the toxic effect of streptozotocin (STZ) in GLUT2(-/-) mice reexpressing either GLUT1 or GLUT2 in their beta cells under the rat insulin promoter (RIPG1 x G2(-/-) and RIPG2 x G2(-/-) mice, respectively). We demonstrated that injection of STZ into RIPG2 x G2(-/-) mice induced hyperglycemia (>20 mM) and an approximately 80% reduction in pancreatic insulin content. In vitro, the viability of RIPG2 x G2(-/-) islets was also strongly reduced. In contrast, STZ did not induce hyperglycemia in RIPG1 x G2(-/-) mice and did not reduce pancreatic insulin content. The viability of in vitro cultured RIPG1 x G2(-/-) islets was also unaffected by STZ. As islets from each type of transgenic mice were functionally indistinguishable, these data strongly support the notion that STZ toxicity toward beta cells depends on the expression of GLUT2.
Keywords
Animals, Blood Glucose/metabolism, Cell Survival/drug effects, Drug Resistance, Gene Expression, Glucose Transporter Type 1, Glucose Transporter Type 2, Humans, Hyperglycemia/chemically induced, Hyperglycemia/genetics, Insulin/genetics, Insulin/metabolism, Islets of Langerhans/cytology, Islets of Langerhans/drug effects, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Monosaccharide Transport Proteins/genetics, Monosaccharide Transport Proteins/metabolism, Promoter Regions, Genetic, Rats, Species Specificity, Streptozocin/toxicity
Pubmed
Web of science
Create date
24/01/2008 13:41
Last modification date
20/08/2019 14:52
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