Metabolic changes after injection of quinolinic acid or 6-hydroxydopamine in the rat striatum: a time-course study using cytochrome oxidase and glycogene phosphorylase a histochemistry.

Détails

ID Serval
serval:BIB_8E5AFF923880
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Metabolic changes after injection of quinolinic acid or 6-hydroxydopamine in the rat striatum: a time-course study using cytochrome oxidase and glycogene phosphorylase a histochemistry.
Périodique
Neurological Research
Auteur(s)
Levivier M., Donaldson D.
ISSN
0161-6412 (Print)
ISSN-L
0161-6412
Statut éditorial
Publié
Date de publication
2000
Peer-reviewed
Oui
Volume
22
Numéro
4
Pages
425-429
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Injection of excitotoxins, such as quinolinic acid (QA), into the striatum has been extensively used as an experimental model of Huntington's disease, while injection of 6-hydroxydopamine (6-OHDA) into the dopaminergic nigrostriatal pathway provides a well established model of Parkinson's disease. In the present study, we have examined the metabolic changes induced by an intrastriatal injection of QA or 6-OHDA using histochemical staining for the metabolic markers cytochrome oxidase (COx) and active glycogene phosphorylase (GPa). Intrastriatal injection of QA produced major changes in COx (decrease of staining) and GPa (increase of staining, except in the core of the lesion where the staining was virtually absent) histochemistry at the level of the striatum and of most of the other basal ganglia nuclei. Although attenuated over time, these changes persisted up to one year after the lesion. On the contrary, after the intrastriatal injection of 6-OHDA (which induces only a partial lesion of the nigrostriatal pathway), we did not observe any remarkable changes in COx or GPa staining. This study illustrates the discrepancies between the morphological changes and metabolic changes that are induced when using these experimental models of neurodegenerative disorders.
Mots-clé
Animals, Autoradiography, Benzazepines/pharmacology, Biological Markers, Corpus Striatum/chemistry, Corpus Striatum/enzymology, Disease Models, Animal, Dopamine Antagonists/pharmacology, Dopamine Uptake Inhibitors/pharmacology, Electron Transport Complex IV/analysis, Glycogen/metabolism, Huntington Disease/enzymology, Male, Mazindol/pharmacology, Microinjections, Nerve Degeneration/chemically induced, Nerve Degeneration/enzymology, Oxidopamine, Parkinson Disease/enzymology, Phosphorylase a/analysis, Quinolinic Acid, Radioligand Assay, Rats, Rats, Sprague-Dawley, Receptors, Dopamine/analysis, Sympatholytics, Tritium/diagnostic use
Pubmed
Web of science
Création de la notice
20/01/2008 18:35
Dernière modification de la notice
03/03/2018 19:18
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