Long synthetic peptides encompassing the Plasmodium falciparum LSA3 are the target of human B and T cells and are potent inducers of B helper, T helper and cytolytic T cell responses in mice

Détails

ID Serval
serval:BIB_8E484C6BD7C3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Long synthetic peptides encompassing the Plasmodium falciparum LSA3 are the target of human B and T cells and are potent inducers of B helper, T helper and cytolytic T cell responses in mice
Périodique
European Journal of Immunology
Auteur(s)
Perlaza  B. L., Sauzet  J. P., Balde  A. T., Brahimi  K., Tall  A., Corradin  G., Druilhe  P.
ISSN
0014-2980 (Print)
Statut éditorial
Publié
Date de publication
07/2001
Volume
31
Numéro
7
Pages
2200-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul
Résumé
We synthesized 17 long synthetic peptides (LSP) spanning the whole 200-kDa Plasmodium falciparum liver stage antigen-3 (LSA3), an antigen that induces protection in chimpanzee, and analyzed their immunogenicity in BALB/c mice and their antigenicity in individuals living in a hyper-endemic malaria area. Our findings show that both specific antibodies and T cell proliferation against most LSA3-LSP develop in malaria-exposed adults. All individuals studied had detectable antibodies against a minimum of 6 and a maximum of 15 polypeptides. It is noteworthy that antibody prevalence and titers were as high against non-repeat as repeat regions. Although the extent of T cell reactivity was lower than that observed for B cells, most of the sequences contained at least one T helper epitope, indicating that the majority of LSA3-LSP contain both B and T cell epitopes within the same sequence. Injection of LSA3-LSP with SBSA2 adjuvant in mice, showed strong immunogenicity for most of them, eliciting both T cell responses and specific antibody production. While all the peptides were immunogenic for B cells, different patterns of T cell responses were induced. These peptides were thus classified in three sets according to the levels of the T cell proliferative and of the IFN-gamma-specific responses. Importantly, antibodies and T cells against some of the LSP were able to recognize LSA3 native protein on P. falciparum sporozoites. Additionally, some LSP (44-119, 1026-1095, 1601-1712) also contained epitopes recognized by H-2(d) class I-restricted T cells. These results led to the identification of numerous domains that are highly antigenic and immunogenic within the LSA3 protein, and underline the value of the LSP approach for vaccine development.
Mots-clé
Adult Animals Antibodies, Protozoan/biosynthesis Antigens, Protozoan/*immunology B-Lymphocytes/*immunology Cells, Cultured Cytotoxicity Tests, Immunologic Epitope Mapping Epitopes, B-Lymphocyte/immunology Epitopes, T-Lymphocyte/immunology Female H-2 Antigens/immunology Humans Interferon Type II/biosynthesis Lymphocyte Activation Malaria Vaccines Malaria, Falciparum/*immunology/therapy Male Mice Mice, Inbred BALB C Peptides/immunology Plasmodium falciparum/*immunology T-Lymphocytes, Cytotoxic/*immunology T-Lymphocytes, Helper-Inducer/*immunology
Pubmed
Web of science
Création de la notice
24/01/2008 15:54
Dernière modification de la notice
20/08/2019 15:52
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