Isolation and characterization of carcinoembryonic antigen (CEA) extracted from normal human colon mucosa.

Détails

ID Serval
serval:BIB_8E360E481D16
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation and characterization of carcinoembryonic antigen (CEA) extracted from normal human colon mucosa.
Périodique
Immunochemistry
Auteur(s)
Fritsche R., Mach J.P.
ISSN
0019-2791 (Print)
ISSN-L
0019-2791
Statut éditorial
Publié
Date de publication
1977
Volume
14
Numéro
2
Pages
119-127
Langue
anglais
Résumé
Carcinoembryonic antigen (CEA) was identified in perchloric acid (PCA)_extract from normal colon mucosa by 2 immunological criteria: a line of identity in double diffusion and a parallel inhibition curve in radioimmunoassay (RIA), both with reference colon carcinoma-CEA (CEA-Tu). The average concentration of CEA in normal colon mucosa (CEA-No) was 35 times lower than in primary large bowel carcinomas and 230 times lower than in metastatic colon or rectum carcinomas. CEA-No was purified from PCA extracts of normal colon mucosa by Sephadex G-200 filtration and immunoadsorbent columns. Purified CEA-No had quatitatively the same inhibition activity in RIA as the British Standard CEA coded 73/601. Purified CEA-No was labelled with 125I. The percentage of binding of labelled CEA-No to a specific goat anti-CEA-Tu antiserum was similar to that of CEA-Tu. Labelled CEA-No could be used as radioactive tracer in RIA as well as labelled CEA-Tu. The physico-chemical properties of purified CEA-Tu as demonstrated by Sepharose 6 B filtration, SDS Polyacrylamide gel analysis and cesium chloride density gradient, were found to be almost identical to those of reference CEA-Tu. Preliminary results showed that CEA-No and CEA-Tu contained the same types of carbohydrates in similar proportions. A rabbit antiserum against CEA-No was obtained which demonstrated the same specificity as conventional anti-CEA-Tu antisera.
Mots-clé
Carcinoembryonic Antigen/isolation & purification, Colon/immunology, Colonic Neoplasms/immunology, Humans, Immune Sera, Immunosorbent Techniques, Mucous Membrane/immunology, Radioimmunoassay
Pubmed
Web of science
Création de la notice
25/04/2013 11:16
Dernière modification de la notice
03/03/2018 19:18
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