A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc.

Détails

ID Serval
serval:BIB_8E2692C0F8F4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A map of protein-protein contacts within the small nuclear RNA-activating protein complex SNAPc.
Périodique
Journal of Biological Chemistry
Auteur(s)
Ma B., Hernandez N.
ISSN
0021-9258[print], 0021-9258[linking]
Statut éditorial
Publié
Date de publication
2001
Volume
276
Numéro
7
Pages
5027-5035
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The nucleation of RNA polymerases I-III transcription complexes is usually directed by distinct multisubunit factors. In the case of the human RNA polymerase II and III small nuclear RNA (snRNA) genes, whose core promoters consist of a proximal sequence element (PSE) and a PSE combined with a TATA box, respectively, the same multisubunit complex is involved in the establishment of RNA polymerase II and III initiation complexes. This factor, the snRNA-activating protein complex or SNAP(c), binds to the PSE of both types of promoters and contains five types of subunits, SNAP190, SNAP50, SNAP45, SNAP43, and SNAP19. SNAP(c) binds cooperatively with both Oct-1, an activator of snRNA promoters, and in the RNA polymerase III snRNA promoters, with TATA-binding protein, which binds to the TATA box located downstream of the PSE. Here we have defined subunit domains required for SNAP(c) subunit-subunit association, and we show that complexes containing little more than the domains mapped here as required for subunit-subunit contacts bind specifically to the PSE. These data provide a detailed map of the subunit-subunit interactions within a multifunctional basal transcription complex.
Mots-clé
Amino Acid Sequence, Amino Acid Substitution, Binding Sites, DNA/metabolism, DNA-Binding Proteins/chemistry, DNA-Binding Proteins/genetics, Humans, Macromolecular Substances, Molecular Sequence Data, Protein Structure, Secondary, Protein Subunits, Proteins/metabolism, RNA-Binding Proteins/metabolism, Response Elements, Sequence Deletion, Transcription Factors/chemistry, Transcription Factors/genetics
Pubmed
Web of science
Création de la notice
21/01/2008 17:33
Dernière modification de la notice
03/03/2018 19:18
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