Degradation of specificity in cytolytic T lymphocyte clones: mouse strain dependence and interstrain transfer of nonspecific cytolysis.

Détails

ID Serval
serval:BIB_8E21956804E9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Degradation of specificity in cytolytic T lymphocyte clones: mouse strain dependence and interstrain transfer of nonspecific cytolysis.
Périodique
European Journal of Immunology
Auteur(s)
Wilson A., Shortman K.
ISSN
0014-2980 (Print)
ISSN-L
0014-2980
Statut éditorial
Publié
Date de publication
1984
Volume
14
Numéro
10
Pages
951-956
Langue
anglais
Résumé
Limiting-dilution culture of murine Ly-2+ T cells with concanavalin A (Con A) and irradiated spleen filler cells produces, with high efficiency, cytolytic T lymphocyte (CTL) clones. With most mouse strains (including CBA and C57BL/6) the specificity of these CTL clones drops after day 6 of culture, so that by day 9 the majority of clones can lyse most murine target cells, whether syngeneic or allogeneic. The rate of specificity degradation and relative target cell preference varies with the mouse strain. Some strains (e.g. BALB/c) do not show this effect and CTL clones remain specific to day 9. Many low natural killer (NK) cell strains (e.g. C57BL/6J.bg) maintain CTL specificity in such cultures, but the correlation between CTL specificity and NK status is not absolute. Growth of BALB/c precursor cells on CBA filler cells leads to specificity degradation in the BALB/c CTL clones; however, the specificity of CBA-derived CTL clones is not maintained by growth on BALB/c fillers. The results suggest that specificity degradation is induced in the developing CTL-clone by factors in the culture environment, perhaps a soluble lymphokine (a differentiation factor) or by an infectious agent (an endogenous mouse virus). Although such CTL specificity loss may render many limiting dilution studies of the CTL specificity repertoire invalid, the problem may be bypassed by an appropriate choice of mouse strain.
Mots-clé
Animals, Cell Line, Clone Cells, Killer Cells, Natural/immunology, Kinetics, Mice, Mice, Inbred Strains, Species Specificity, Spleen, T-Lymphocytes, Cytotoxic/immunology
Pubmed
Web of science
Création de la notice
10/04/2013 10:54
Dernière modification de la notice
20/08/2019 14:52
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