Construction and electrophoretic migration of single-stranded DNA knots and catenanes.

Details

Serval ID
serval:BIB_8E12231FD29E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Construction and electrophoretic migration of single-stranded DNA knots and catenanes.
Journal
Nucleic Acids Research
Author(s)
Bucka A., Stasiak A.
ISSN
1362-4962[electronic], 0305-1048[linking]
Publication state
Published
Issued date
03/2002
Volume
30
Number
6
Pages
e24
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In recent years there has been growing interest in the question of how the particular topology of polymeric chains affects their overall dimensions and physical behavior. The majority of relevant studies are based on numerical simulation methods or analytical treatment; however, both these approaches depend on various assumptions and simplifications. Experimental verification is clearly needed but was hampered by practical difficulties in obtaining preparative amounts of knotted or catenated polymers with predefined topology and precisely set chain length. We introduce here an efficient method of production of various single-stranded DNA knots and catenanes that have the same global chain length. We also characterize electrophoretic migration of the produced single-stranded DNA knots and catenanes with increasing complexity.
Keywords
DNA, Single-Stranded/chemistry, Electrophoresis, Polyacrylamide Gel/methods, Models, Genetic, Nucleic Acid Conformation, Oligodeoxyribonucleotides/chemistry
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 11:36
Last modification date
20/08/2019 15:52
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