Construction and electrophoretic migration of single-stranded DNA knots and catenanes.

Détails

ID Serval
serval:BIB_8E12231FD29E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Construction and electrophoretic migration of single-stranded DNA knots and catenanes.
Périodique
Nucleic Acids Research
Auteur(s)
Bucka A., Stasiak A.
ISSN
1362-4962[electronic], 0305-1048[linking]
Statut éditorial
Publié
Date de publication
03/2002
Volume
30
Numéro
6
Pages
e24
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
In recent years there has been growing interest in the question of how the particular topology of polymeric chains affects their overall dimensions and physical behavior. The majority of relevant studies are based on numerical simulation methods or analytical treatment; however, both these approaches depend on various assumptions and simplifications. Experimental verification is clearly needed but was hampered by practical difficulties in obtaining preparative amounts of knotted or catenated polymers with predefined topology and precisely set chain length. We introduce here an efficient method of production of various single-stranded DNA knots and catenanes that have the same global chain length. We also characterize electrophoretic migration of the produced single-stranded DNA knots and catenanes with increasing complexity.
Mots-clé
DNA, Single-Stranded/chemistry, Electrophoresis, Polyacrylamide Gel/methods, Models, Genetic, Nucleic Acid Conformation, Oligodeoxyribonucleotides/chemistry
Pubmed
Web of science
Création de la notice
24/01/2008 11:36
Dernière modification de la notice
03/03/2018 19:17
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