The SV40 enhancer: transcriptional regulation through a hierarchy of combinatorial interactions

Détails

ID Serval
serval:BIB_8DC3B111ABDE
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
The SV40 enhancer: transcriptional regulation through a hierarchy of combinatorial interactions
Périodique
Seminars in Virology
Auteur(s)
Herr W.
ISSN
1044-5773
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
4
Numéro
1
Pages
3-13
Langue
anglais
Résumé
The simian DNA tumor virus SV40 regulates synthesis of early and late viral transcripts from two divergent and overlapping promoters. The SV40 early promoter exhibits all the hallmarks of eukaryotic promoters, containing elements that function when positioned near the start site of transcription and other positionally flexible elements that constitute a transcriptional enhancer. The architecture of the SV40 enhancer has been elucidated by four complementary approaches: (1) saturation mutagenesis in vitro; (2) viral genetic selection in vivo; (3) synthetic multimerization of its component parts; and (4) detailed protein binding studies. The SV40 enhancer represents a cluster of cell-specific regulatory elements that cooperate with one another in a functional hierarchy. The basic units of enhancer structure, enhansons, are the building blocks and represent protein binding sites. The functional units are proto-enhancers, which consist of one or more enhansons. Proto-enhancers serve as regulatory modules that can cooperate with one another or duplicates of themselves in flexible arrangements to create a potent enhancer.
Mots-clé
enhancer, enhanson, proto-enhancer, SV40, transcription
Web of science
Création de la notice
15/03/2010 16:14
Dernière modification de la notice
20/08/2019 15:51
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