Inhibition of angiotensin converting enzyme activity in cultured endothelial cells by hypoxia

Détails

ID Serval
serval:BIB_8DB6080E4D74
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inhibition of angiotensin converting enzyme activity in cultured endothelial cells by hypoxia
Périodique
Journal of Clinical Investigation
Auteur(s)
Stalcup  S. A., Lipset  J. S., Woan  J. M., Leuenberger  P., Mellins  R. B.
ISSN
0021-9738 (Print)
Statut éditorial
Publié
Date de publication
05/1979
Volume
63
Numéro
5
Pages
966-76
Notes
Journal Article
Research Support, U.S. Gov't, P.H.S.
Retracted Publication --- Old month value: May
Résumé
Endothelial cells in tissue culture degrade bradykinin and convert angiotensin I to angiotensin II. These are both functions of a single dipeptidyl hydrolase, angiotensin converting enzyme. Monolayer cultures were prepared from human, rabbit, pig, and calf vessels. Angiotensin converting enzyme activity was assessed by adding either bradykinin or angiotensin I to the cells in culture flasks, and measuring residual peptide over time by radioimmunoassay. Peptide degradation was inhibited by the specific converting enzyme inhibitor, SQ 20881. The flasks were equilibrated with varying hypoxic gas mixtures: hypoxia rapidly (less than 2 min) decreased enzyme activity and room air restored it as rapidly. The extent to which activity was reduced was a direct function of PO2 (r = 0.93, P less than 0.001), and there was no enzyme activity below a PO2 of 30 mm Hg. Four preparations were studied with respect to decrease in enzyme activity by hypoxia: (a) intact cells in monolayer, (b) sonicated cells, (c) sonicated cells from which converting enzyme was partially dissolved by a detergent, and (d) purified converting enzyme. Hypoxia had progressively less of an inhibiting effect on the enzyme activity of the preparations as the degree of cell integrity decreased. Hypoxia inhibits angiotensin converting enzyme activity in cultured endothelial cells, but the effect of hypoxia is not on the enzyme per se, but appears to be a unique characteristic of the endothelial cell.
Mots-clé
Animals Anoxia/*enzymology/pathology Aorta/cytology Cattle Cells, Cultured Endothelium/*enzymology/ultrastructure Histocytochemistry Humans Lung/cytology Microscopy, Electron, Scanning Peptidyl-Dipeptidase A/*metabolism Rabbits Rats Umbilical Cord/cytology
Pubmed
Web of science
Création de la notice
25/01/2008 10:50
Dernière modification de la notice
03/03/2018 19:16
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