Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances.

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ID Serval
serval:BIB_8DB444261E9B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances.
Périodique
NMR in Biomedicine
Auteur(s)
van Heeswijk R.B., Morgenthaler F.D., Xin L., Gruetter R.
ISSN
1099-1492 (Electronic)
ISSN-L
0952-3480
Statut éditorial
Publié
Date de publication
04/2010
Volume
23
Numéro
3
Pages
270-276
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish. PDF type: Research Article
Résumé
We have recently shown that at isotopic steady state (13)C NMR can provide a direct measurement of glycogen concentration changes, but that the turnover of glycogen was not accessible with this protocol. The aim of the present study was to design, implement and apply a novel dual-tracer infusion protocol to simultaneously measure glycogen concentration and turnover. After reaching isotopic steady state for glycogen C1 using [1-(13)C] glucose administration, [1,6-(13)C(2)] glucose was infused such that isotopic steady state was maintained at the C1 position, but the C6 position reflected (13)C label incorporation. To overcome the large chemical shift displacement error between the C1 and C6 resonances of glycogen, we implemented 2D gradient based localization using the Fourier series window approach, in conjunction with time-domain analysis of the resulting FIDs using jMRUI. The glycogen concentration of 5.1 +/- 1.6 mM measured from the C1 position was in excellent agreement with concomitant biochemical determinations. Glycogen turnover measured from the rate of label incorporation into the C6 position of glycogen in the alpha-chloralose anesthetized rat was 0.7 micromol/g/h.
Mots-clé
Animals, Brain/metabolism, Carbon/metabolism, Carbon Isotopes, Computer Simulation, Fourier Analysis, Glucose/metabolism, Glycogen/metabolism, Magnetic Resonance Spectroscopy, Rats, Rats, Sprague-Dawley, Time Factors
Pubmed
Web of science
Création de la notice
04/08/2010 16:28
Dernière modification de la notice
06/12/2018 16:39
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