Reggie-1/Flotillin-2 regulates integrin trafficking and focal adhesion turnover via Rab11a.

Détails

ID Serval
serval:BIB_8D81C3B356A6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reggie-1/Flotillin-2 regulates integrin trafficking and focal adhesion turnover via Rab11a.
Périodique
European Journal of Cell Biology
Auteur(s)
Hülsbusch N., Solis G.P., Katanaev V.L., Stuermer C.A.
ISSN
1618-1298 (Electronic)
ISSN-L
0171-9335
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
94
Numéro
11
Pages
531-545
Langue
anglais
Résumé
Reggies/flotillins are implicated in trafficking of membrane proteins to their target sites and in the regulation of the Rab11a-dependent targeted recycling of E-cadherin to adherens junctions (AJs). Here we demonstrate a function of reggies in focal adhesion (FA) formation and α5- and β1-integrin recycling to FAs. Downregulation of reggie-1 in HeLa and A431 cells by siRNA and shRNA increased the number of FAs, impaired their distribution and modified FA turnover. This was coupled to enhanced focal adhesion kinase (FAK) and Rac1 signaling and gain in plasma membrane motility. Wild type and constitutively-active (CA) Rab11a rescued the phenotype (normal number of FAs) whereas dominant-negative (DN) Rab11a mimicked the loss-of-reggie phenotype in control cells. That reggie-1 affects integrin trafficking emerged from the faster loss of internalized antibody-labeled β1-integrin in reggie-deficient cells. Moreover, live imaging using TIRF microscopy revealed vesicles containing reggie-1 and α5- or β1-integrin, trafficking close to the substrate-near membrane and making kiss-and-run contacts with FAs. Thus, reggie-1 in interaction with Rab11a controls Rac1 and FAK activation and coordinates the targeted recycling of α5- and β1-integrins to FAs to regulate FA formation and membrane dynamics.
Mots-clé
Antigens, CD29/metabolism, Focal Adhesion Protein-Tyrosine Kinases/metabolism, Focal Adhesions/metabolism, HeLa Cells, Humans, Integrin alpha5/metabolism, Membrane Proteins/genetics, Membrane Proteins/metabolism, Protein Transport, rab GTP-Binding Proteins/metabolism, rac1 GTP-Binding Protein/metabolism
Pubmed
Web of science
Création de la notice
03/01/2016 16:35
Dernière modification de la notice
20/08/2019 14:51
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