Oligotyping of HLA-A2, -A3, and -B44 subtypes. Detection of subtype incompatibilities between patients and their serologically matched unrelated bone marrow donors
Details
Serval ID
serval:BIB_8D747B76EC2C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Oligotyping of HLA-A2, -A3, and -B44 subtypes. Detection of subtype incompatibilities between patients and their serologically matched unrelated bone marrow donors
Journal
Human Immunology
ISSN
0198-8859 (Print)
Publication state
Published
Issued date
11/1994
Volume
41
Number
3
Pages
207-15
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
We have set up a simple PCR-SSO oligotyping procedure that is able to discriminate ten HLA-A2 (2 PCR/11 probes), two HLA-A3 (1 PCR/1 probe), and two HLA-B44 subtypes (1 PCR/2 probes). The frequency of these subtypes has been determined in a large panel of local blood donors and leukemic patients in combination with their unrelated potential donors. A*0201 and A*0301 were the predominant subtypes (> 95%) for A2 and A3, respectively. B*4402 occurred twice as frequently as B*4403. A2 and B44 subtype mismatches were analyzed in a group of 30 patients and their 116 unrelated potential donors who were matched serologically (low-stringency matching: AB without splits, DR1-10). For seven patients (23%) at least one A2- or B44-subtype-mismatched donor was found. For two of these patients (7%), the subtype-mismatched donor would have been considered as compatible on the basis of high stringency matching (AB splits, DRB1 subtypes, DRB3/B5). For one patient of Mediterranean origin, all five donors recruited from a north European registry (matched with high stringency) appeared to be subtype incompatible (A*0201/A*0205). The rather low percentage of A2- and B4-subtype mismatches in DRB1/B3/B5 matched combinations confirms the significance of linkage disequilibria of HLA antigens. Because unrelated donor selection is done through international registries, however, class I subtyping might be necessary when individuals originate from different geographic areas.
Keywords
Base Sequence
Bone Marrow Transplantation/*immunology
European Continental Ancestry Group/genetics
HLA Antigens/*genetics
HLA-A2 Antigen/genetics
HLA-A3 Antigen/genetics
HLA-B Antigens/genetics
Humans
Molecular Sequence Data
Polymerase Chain Reaction
*Polymorphism, Genetic
Pubmed
Web of science
Create date
28/01/2008 12:33
Last modification date
20/08/2019 15:51