Oligotyping of HLA-A2, -A3, and -B44 subtypes. Detection of subtype incompatibilities between patients and their serologically matched unrelated bone marrow donors

Détails

ID Serval
serval:BIB_8D747B76EC2C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Oligotyping of HLA-A2, -A3, and -B44 subtypes. Detection of subtype incompatibilities between patients and their serologically matched unrelated bone marrow donors
Périodique
Human Immunology
Auteur(s)
Tiercy  J. M., Djavad  N., Rufer  N., Speiser  D. E., Jeannet  M., Roosnek  E.
ISSN
0198-8859 (Print)
Statut éditorial
Publié
Date de publication
11/1994
Volume
41
Numéro
3
Pages
207-15
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Résumé
We have set up a simple PCR-SSO oligotyping procedure that is able to discriminate ten HLA-A2 (2 PCR/11 probes), two HLA-A3 (1 PCR/1 probe), and two HLA-B44 subtypes (1 PCR/2 probes). The frequency of these subtypes has been determined in a large panel of local blood donors and leukemic patients in combination with their unrelated potential donors. A*0201 and A*0301 were the predominant subtypes (> 95%) for A2 and A3, respectively. B*4402 occurred twice as frequently as B*4403. A2 and B44 subtype mismatches were analyzed in a group of 30 patients and their 116 unrelated potential donors who were matched serologically (low-stringency matching: AB without splits, DR1-10). For seven patients (23%) at least one A2- or B44-subtype-mismatched donor was found. For two of these patients (7%), the subtype-mismatched donor would have been considered as compatible on the basis of high stringency matching (AB splits, DRB1 subtypes, DRB3/B5). For one patient of Mediterranean origin, all five donors recruited from a north European registry (matched with high stringency) appeared to be subtype incompatible (A*0201/A*0205). The rather low percentage of A2- and B4-subtype mismatches in DRB1/B3/B5 matched combinations confirms the significance of linkage disequilibria of HLA antigens. Because unrelated donor selection is done through international registries, however, class I subtyping might be necessary when individuals originate from different geographic areas.
Mots-clé
Base Sequence Bone Marrow Transplantation/*immunology European Continental Ancestry Group/genetics HLA Antigens/*genetics HLA-A2 Antigen/genetics HLA-A3 Antigen/genetics HLA-B Antigens/genetics Humans Molecular Sequence Data Polymerase Chain Reaction *Polymorphism, Genetic
Pubmed
Web of science
Création de la notice
28/01/2008 12:33
Dernière modification de la notice
03/03/2018 19:16
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