Bcl-2 expression in breast cancer: a comparative study at the mRNA and protein level.

Détails

ID Serval
serval:BIB_8D5492A75348
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bcl-2 expression in breast cancer: a comparative study at the mRNA and protein level.
Périodique
Anticancer research
Auteur(s)
Martínez-Arribas F., Alvarez T., Del Val G., Martín-Garabato E., Núñez-Villar M.J., Lucas R., Sánchez J., Tejerina A., Schneider J.
ISSN
0250-7005 (Print)
ISSN-L
0250-7005
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
27
Numéro
1A
Pages
219-222
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Résumé
Bcl-2 is one of the most important antiapoptotic genes. Although it facilitates the survival of tumor cells, its expression has been consistently associated with a better prognosis for breast cancer. Virtually all studies on Bcl-2 conducted in breast cancer have been carried out by means of immunohistochemistry. The aim of this study was to examine for the first time the expression of Bcl-2 in a series of human breast cancer, both at the mRNA and protein level.
One hundred samples from previously untreated human breast cancers were used; Bcl-2 expression was determined both by means of immunohistochemistry using the bcl-2/100/D5 monoclonal antibody and differential RT-PCR. Additionally, the expression of hormone receptors (ER and PR), c-erb-B2, p53 and the proliferation-associated Ki-67 antigen were also studied by means of immunohistochemistry as part of the standard pathological workup.
Any degree of immunohistochemical staining correlated significantly and inversely with c-erb-B2 expression (p = 0.0008), nuclear grade 3 (p = 0.0015), a Ki-67 labeling index > 10% (p = 0.02) and tumor size (p = 0.048), and in a direct fashion with estrogen (p = 0.0003) and progesterone receptor expression (p = 0.0002). mRNA expression of the Bcl-2 gene showed a significant inverse correlation with c-erb-B2 (p = 0.016) and p53 (p = 0.014) expression, as well as with a nuclear grade 3 (p = 0.006), and a direct correlation with estrogen (p = 0.0004) and progesterone (p = 0.001) receptor expression, as well as with nodal invasion (p = 0.04).
The study of Bcl-2 expression in breast cancer by means of either immunohistochemistry or RT-PCR yields very similar results. In spite of its role opposing tumor cell death, Bcl-2 is associated with biological features of the tumors which define a better intrinsic prognosis, such as hormone receptor expression, low proliferation and absence of c-erb-B2 and mutant p53 expression. This may in great part explain why Bcl-2 expression has been invariably found to correlate with a better prognosis of breast cancer.

Mots-clé
Breast Neoplasms/genetics, Breast Neoplasms/metabolism, Breast Neoplasms/pathology, Humans, Immunohistochemistry, Neoplasm Staging, Proto-Oncogene Proteins c-bcl-2/biosynthesis, Proto-Oncogene Proteins c-bcl-2/genetics, RNA, Messenger/biosynthesis, RNA, Messenger/genetics, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
13/02/2018 10:26
Dernière modification de la notice
06/03/2018 7:26
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