Chloroplastic phosphoadenosine phosphosulfate metabolism regulates basal levels of the prohormone jasmonic acid in Arabidopsis leaves.

Détails

ID Serval
serval:BIB_8D4BAE61CE94
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Chloroplastic phosphoadenosine phosphosulfate metabolism regulates basal levels of the prohormone jasmonic acid in Arabidopsis leaves.
Périodique
Plant Physiology
Auteur(s)
Rodríguez V.M., Chételat A., Majcherczyk P., Farmer E.E.
ISSN
1532-2548[electronic], 0032-0889[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
152
Numéro
3
Pages
1335-1345
Langue
anglais
Résumé
Levels of the enzymes that produce wound response mediators have to be controlled tightly in unwounded tissues. The Arabidopsis (Arabidopsis thaliana) fatty acid oxygenation up-regulated8 (fou8) mutant catalyzes high rates of alpha -linolenic acid oxygenation and has higher than wild-type levels of the alpha -linolenic acid-derived wound response mediator jasmonic acid (JA) in undamaged leaves. fou8 produces a null allele in the gene SAL1 (also known as FIERY1 or FRY1). Overexpression of the wild-type gene product had the opposite effect of the null allele, suggesting a regulatory role of SAL1 acting in JA synthesis. The biochemical phenotypes in fou8 were complemented when the yeast (Saccharomyces cerevisiae) sulfur metabolism 3'(2'), 5'-bisphosphate nucleotidase MET22 was targeted to chloroplasts in fou8. The data are consistent with a role of SAL1 in the chloroplast-localized dephosphorylation of 3'-phospho-5'-adenosine phosphosulfate to 5'-adenosine phosphosulfate or in a closely related reaction (e.g. 3',5'-bisphosphate dephosphorylation). Furthermore, the fou8 phenotype was genetically suppressed in a triple mutant (fou8 apk1 apk2) affecting chloroplastic 3'-phospho-5'-adenosine phosphosulfate synthesis. These results show that a nucleotide component of the sulfur futile cycle regulates early steps of JA production and basal JA levels.
Mots-clé
Arabidopsis/genetics, Arabidopsis/metabolism, Arabidopsis Proteins/genetics, Arabidopsis Proteins/metabolism, Chloroplasts/metabolism, Cyclopentanes/metabolism, DNA, Plant/genetics, Gene Expression Regulation, Plant, Mutation, Oxylipins/metabolism, Phosphoadenosine Phosphosulfate/metabolism, Phosphoric Monoester Hydrolases/genetics, Phosphoric Monoester Hydrolases/metabolism, Plant Growth Regulators/metabolism, Plant Leaves/metabolism, Plants, Genetically Modified/genetics, Plants, Genetically Modified/metabolism
Pubmed
Web of science
Création de la notice
13/01/2010 14:57
Dernière modification de la notice
03/03/2018 19:15
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