Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor.

Détails

Ressource 1Télécharger: BIB_8D169A5DD22B.P001.pdf (660.82 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_8D169A5DD22B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Simultaneous Optical Recording in Multiple Cells by Digital Holographic Microscopy of Chloride Current Associated to Activation of the Ligand-Gated Chloride Channel GABA(A) Receptor.
Périodique
Plos One
Auteur(s)
Jourdain P., Boss D., Rappaz B., Moratal C., Hernandez M.C., Depeursinge C., Magistretti P.J., Marquet P.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2012
Peer-reviewed
Oui
Volume
7
Numéro
12
Pages
e51041
Langue
anglais
Notes
Publication types: Journal Article
Résumé
Chloride channels represent a group of targets for major clinical indications. However, molecular screening for chloride channel modulators has proven to be difficult and time-consuming as approaches essentially rely on the use of fluorescent dyes or invasive patch-clamp techniques which do not lend themselves to the screening of large sets of compounds. To address this problem, we have developed a non-invasive optical method, based on digital holographic microcopy (DHM), allowing monitoring of ion channel activity without using any electrode or fluorescent dye. To illustrate this approach, GABA(A) mediated chloride currents have been monitored with DHM. Practically, we show that DHM can non-invasively provide the quantitative determination of transmembrane chloride fluxes mediated by the activation of chloride channels associated with GABA(A) receptors. Indeed through an original algorithm, chloride currents elicited by application of appropriate agonists of the GABA(A) receptor can be derived from the quantitative phase signal recorded with DHM. Finally, chloride currents can be determined and pharmacologically characterized non-invasively simultaneously on a large cellular sampling by DHM.
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/12/2012 9:32
Dernière modification de la notice
20/08/2019 14:51
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