Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.

Détails

ID Serval
serval:BIB_8CE3CE090C29
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters.
Périodique
Genes and Development
Auteur(s)
Schramm L., Pendergrast P.S., Sun Y., Hernandez N.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
10/2000
Volume
14
Numéro
20
Pages
2650-2663
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Transcription initiation at RNA polymerase III promoters requires transcription factor IIIB (TFIIIB), an activity that binds to RNA polymerase III promoters, generally through protein-protein contacts with DNA binding factors, and directly recruits RNA polymerase III. Saccharomyces cerevisiae TFIIIB is a complex of three subunits, TBP, the TFIIB-related factor BRF, and the more loosely associated polypeptide beta("). Although human homologs for two of the TFIIIB subunits, the TATA box-binding protein TBP and the TFIIB-related factor BRF, have been characterized, a human homolog of yeast B(") has not been described. Moreover, human BRF, unlike yeast BRF, is not universally required for RNA polymerase III transcription. In particular, it is not involved in transcription from the small nuclear RNA (snRNA)-type, TATA-containing, RNA polymerase III promoters. Here, we characterize two novel activities, a human homolog of yeast B("), which is required for transcription of both TATA-less and snRNA-type RNA polymerase III promoters, and a factor equally related to human BRF and TFIIB, designated BRFU, which is specifically required for transcription of snRNA-type RNA polymerase III promoters. Together, these results contribute to the definition of the basal RNA polymerase III transcription machinery and show that two types of TFIIIB activities, with specificities for different classes of RNA polymerase III promoters, have evolved in human cells.
Mots-clé
Amino Acid Sequence, Cloning, Molecular, Humans, Molecular Sequence Data, Promoter Regions, Genetic, RNA Polymerase III/genetics, RNA Polymerase III/metabolism, RNA, Viral/genetics, Ribonucleoprotein, U4-U6 Small Nuclear/genetics, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, TATA Box, TATA-Binding Protein Associated Factors, Transcription Factor TFIIIB, Transcription Factors/genetics, Transcription Factors/metabolism, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
21/01/2008 16:33
Dernière modification de la notice
20/08/2019 14:51
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