Link between genotype and antimicrobial resistance in bovine mastitis-related Staphylococcus aureus strains, determined by comparing Swiss and French isolates from the Rhône Valley.

Détails

ID Serval
serval:BIB_8CC32A7B5B1F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Link between genotype and antimicrobial resistance in bovine mastitis-related Staphylococcus aureus strains, determined by comparing Swiss and French isolates from the Rhône Valley.
Périodique
Applied and Environmental Microbiology
Auteur(s)
Sakwinska O., Morisset D., Madec J.Y., Waldvogel A., Moreillon P., Haenni M.
ISSN
1098-5336 (Electronic)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2011
Volume
77
Numéro
10
Pages
3428-3432
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Staphylococcus aureus is a major bovine mastitis pathogen. Although the reported antimicrobial resistance was generally low, the emergence of new genetic clusters in bovine mastitis requires examination of the link between antimicrobial resistance and genotypes. Here, amplified fragment length polymorphism (AFLP) profiles and standard antimicrobial resistance profiles were determined in order to characterize a total of 343 S. aureus cow mastitis isolates from two geographically close regions of Switzerland and France. AFLP profiles revealed similar population compositions in the two regions, with 4 major clusters (C8, C20, C97, and C151), but the proportions of isolates in each cluster significantly diverged between the two countries (P = 9.2 × 10⁻⁹). Antimicrobial resistance was overall low (< 5% resistance to all therapeutically relevant molecules), with the exception of penicillin resistance, which was detected in 26% of the isolates. Penicillin resistance proportions differed between clusters, with only 1 to 2% of resistance associated with C20 and C151 and up to 70% associated with bovine C97. The prevalence of C20 and C8 was unexpectedly high and requires further investigation into the mechanism of adaptation to the bovine host. The strong association of penicillin resistance with few clusters highlights the fact that the knowledge of local epidemiology is essential for rational choices of antimicrobial treatment in the absence of susceptibility testing. Taken together, these observations argue in favor of more routine scrutiny of antimicrobial resistance and antibiotic-resistant clones in cattle and the farm environment.
Mots-clé
Amplified Fragment Length Polymorphism Analysis, Animals, Bacterial Typing Techniques, Cattle, Cluster Analysis, Drug Resistance, Bacterial, Female, France, Genotype, Mastitis, Bovine/microbiology, Microbial Sensitivity Tests, Molecular Typing, Staphylococcal Infections/microbiology, Staphylococcal Infections/veterinary, Staphylococcus aureus/drug effects, Staphylococcus aureus/genetics, Switzerland
Pubmed
Web of science
Open Access
Oui
Création de la notice
06/12/2011 16:38
Dernière modification de la notice
08/05/2019 21:46
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