Immunodetection of endogenous opioid peptides in human brain tumors and associated cyst fluids

Détails

ID Serval
serval:BIB_8CB3B79633CC
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Immunodetection of endogenous opioid peptides in human brain tumors and associated cyst fluids
Périodique
Cancer Research
Auteur(s)
Gustin  T., Bachelot  T., Verna  J. M., Molin  L. F., Brunet  J. F., Berger  F. R., Benabid  A. L.
ISSN
0008-5472
Statut éditorial
Publié
Date de publication
10/1993
Peer-reviewed
Oui
Volume
53
Numéro
19
Pages
4715-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct 1
Résumé
The antitumorigenic effects of endogenous opioid peptides and their presence in extracerebral tumors are well documented. In this study, methionine-enkephaline (met-enkephalin) was measured by radioimmunoassay in 108 glial and nonglial brain tumors and in 44 associated cyst fluids. By immunohistochemistry, the distribution of the peptide and its precursor, preproenkephalin A, was also analyzed. Met-enkephalin and preproenkephalin were detected in the cytoplasm and cell processes of all tumors. Moreover, for neuroectodermal tumors (i.e., gliomas, gangliogliomas, and dysembryoplastic neuroepithelial tumors), a strong inverse correlation (P < 0.0001) was observed between the met-enkephalin levels and the degree of malignancy (242.9, 148.3, 55.3, and 30.3 pg/mg protein for grade 1, 2, 3, and 4, respectively). When compared to normal tissue, this differential expression mainly results from a decrease in the opioid peptide content in high-grade neuroectodermal tumors. Meningiomas and cerebral metastases displayed low met-enkephalin levels, similar to those of grade 4 neuroectodermal tumors. Large amounts of met-enkephalin were found in all cyst fluids. These data suggest that the endogenous opioid system is an integral component of brain tumors and that met-enkephalin may represent a useful malignancy marker in neuroectodermal tumors.
Mots-clé
Brain Neoplasms/*chemistry/classification/*pathology/surgery Cysts/*chemistry/classification/*pathology/surgery Enkephalin, Methionine/*analysis Enkephalins/*analysis Glial Fibrillary Acidic Protein/analysis Glioma/*chemistry/classification/*pathology/surgery Humans Immunohistochemistry Protein Precursors/*analysis Radioimmunoassay
Pubmed
Web of science
Création de la notice
06/02/2008 10:44
Dernière modification de la notice
03/03/2018 19:14
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