Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions

Détails

ID Serval
serval:BIB_8CB08A11DD9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions
Périodique
Diagnostic Microbiology and Infectious Disease
Auteur(s)
Welti  M., Jaton  K., Altwegg  M., Sahli  R., Wenger  A., Bille  J.
ISSN
0732-8893 (Print)
Statut éditorial
Publié
Date de publication
02/2003
Volume
45
Numéro
2
Pages
85-95
Notes
Journal Article --- Old month value: Feb
Résumé
Atypical pathogens such as Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae are an important cause of community-acquired pneumonia. The available detection methods (culture and serology) either lack sensitivity or give only a retrospective diagnosis. In order to improve their detection and quantification in respiratory samples, a real-time multiplex PCR, performed in two separate reactions, was developed for these three pathogens. The comparison of multiplex real-time and conventional PCR assay on 73 respiratory specimens showed an overall agreement of 98.3%, corresponding to 95.8%, 100% and 100% agreement for C. pneumoniae, L. pneumophila and M. pneumoniae, respectively. Clinical application of this multiplex real-time PCR was done on 40 respiratory samples from 38 patients with respiratory tract infections. Of 19 serology-positive patients, 14 were confirmed by the multiplex real-time PCR to be infected by either one of the three pathogens. All samples from serology-negative patients were negative with the multiplex real-time PCR.
Mots-clé
Biotechnology Chlamydophila Infections/diagnosis Chlamydophila pneumoniae/genetics/*isolation & purification DNA, Bacterial/analysis Humans Legionella pneumophila/genetics/*isolation & purification Legionnaires' Disease/diagnosis Mycoplasma pneumoniae/genetics/*isolation & purification Pneumonia, Bacterial/*diagnosis Pneumonia, Mycoplasma/diagnosis Polymerase Chain Reaction/*methods Respiratory System/microbiology/secretion Retrospective Studies Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
24/01/2008 21:51
Dernière modification de la notice
03/03/2018 19:14
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