A Surface-Induced Asymmetric Program Promotes Tissue Colonization by Pseudomonas aeruginosa.

Détails

ID Serval
serval:BIB_8C7565359992
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A Surface-Induced Asymmetric Program Promotes Tissue Colonization by Pseudomonas aeruginosa.
Périodique
Cell host & microbe
Auteur(s)
Laventie B.J., Sangermani M., Estermann F., Manfredi P., Planes R., Hug I., Jaeger T., Meunier E., Broz P., Jenal U.
ISSN
1934-6069 (Electronic)
ISSN-L
1931-3128
Statut éditorial
Publié
Date de publication
09/01/2019
Peer-reviewed
Oui
Volume
25
Numéro
1
Pages
140-152.e6
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The opportunistic human pathogen Pseudomonas aeruginosa effectively colonizes host epithelia using pili as primary adhesins. Here we uncover a surface-specific asymmetric virulence program that enhances P. aeruginosa host colonization. We show that when P. aeruginosa encounters surfaces, the concentration of the second messenger c-di-GMP increases within a few seconds. This leads to surface adherence and virulence induction by stimulating pili assembly through activation of the c-di-GMP receptor FimW. Surface-attached bacteria divide asymmetrically to generate a piliated, surface-committed progeny (striker) and a flagellated, motile offspring that leaves the surface to colonize distant sites (spreader). Cell differentiation is driven by a phosphodiesterase that asymmetrically positions to the flagellated pole, thereby maintaining c-di-GMP levels low in the motile offspring. Infection experiments demonstrate that cellular asymmetry strongly boosts infection spread and tissue damage. Thus, P. aeruginosa promotes surface colonization and infection transmission through a cooperative virulence program that we termed Touch-Seed-and-Go.
Mots-clé
A549 Cells, Adhesins, Bacterial/metabolism, Apoptosis, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Biofilms/growth & development, Carrier Proteins, Cell Differentiation, Cyclic GMP/analogs & derivatives, Cyclic GMP/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Fimbriae, Bacterial/metabolism, Gene Deletion, Gene Expression Regulation, Bacterial, Homologous Recombination, Humans, Mutagenesis, Site-Directed, Phosphoric Diester Hydrolases/metabolism, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/cytology, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/metabolism, Pseudomonas aeruginosa/pathogenicity, Virulence, Pseudomonas aeruginosa, asymmetric division, c-di-GMP, flagella, second messenger, surface sensing, tissue colonization, type IV pili, virulence
Pubmed
Web of science
Création de la notice
20/01/2019 17:06
Dernière modification de la notice
20/08/2019 15:50
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