Role of Na+-K+-ATPase in insulin-induced lactate release by skeletal muscle.

Détails

ID Serval
serval:BIB_8C1B6AAAA456
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Role of Na+-K+-ATPase in insulin-induced lactate release by skeletal muscle.
Périodique
American Journal of Physiology. Endocrinology and Metabolism
Auteur(s)
Novel-Chaté V., Rey V., Chioléro R., Schneiter P., Leverve X., Jéquier E., Tappy L.
ISSN
0193-1849 (Print)
ISSN-L
0193-1849
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
280
Numéro
2
Pages
E296-E300
Langue
anglais
Résumé
Hyperinsulinemia increases lactate release by various organs and tissues. Whereas it has been shown that aerobic glycolysis is linked to Na+-K+-ATPase activity, we hypothesized that stimulation by insulin of skeletal muscle Na+-K+-ATPase is responsible for increased muscle lactate production. To test this hypothesis, we assessed muscle lactate release in healthy volunteers from the [13C]lactate concentration in the effluent dialysates of microdialysis probes inserted into the tibialis anterior muscles on both sides and infused with solutions containing 5 mmol/l [U-13C]glucose. On one side, the microdialysis probe was intermittently infused with the same solution additioned with 2.10(-5) M ouabain. In the basal state, [13C]lactate concentration in the dialysate was not affected by ouabain. During a euglycemic-hyperinsulinemic clamp, [13C]lactate concentration increased by 135% in the dialysate without ouabain, and this stimulation was nearly entirely reversed by ouabain (56% inhibition compared with values in the dialysate collected from the contralateral probe). These data indicate that insulin stimulates muscle lactate release by activating Na+-K+-ATPase in healthy humans.
Mots-clé
Adult, Blood Glucose/analysis, Enzyme Inhibitors/pharmacology, Humans, Insulin/blood, Insulin/pharmacology, Lactic Acid/antagonists & inhibitors, Lactic Acid/blood, Microdialysis, Muscle, Skeletal/metabolism, Osmolar Concentration, Ouabain/pharmacology, Sodium-Potassium-Exchanging ATPase/physiology, Time Factors
Pubmed
Web of science
Création de la notice
24/01/2008 14:36
Dernière modification de la notice
03/03/2018 19:13
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