Comparison of inflammation, organ damage, and oxidant stress induced by Salmonella enterica serovar Muenchen flagellin and serovar Enteritidis lipopolysaccharide

Détails

ID Serval
serval:BIB_8C1056C9E5C6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparison of inflammation, organ damage, and oxidant stress induced by Salmonella enterica serovar Muenchen flagellin and serovar Enteritidis lipopolysaccharide
Périodique
Infection and Immunity
Auteur(s)
Liaudet  L., Murthy  K. G., Mabley  J. G., Pacher  P., Soriano  F. G., Salzman  A. L., Szabo  C.
ISSN
0019-9567 (Print)
Statut éditorial
Publié
Date de publication
01/2002
Volume
70
Numéro
1
Pages
192-8
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan
Résumé
Gram-negative sepsis is related to the activation of interconnected inflammatory cascades in response to bacteria and their products. Recent work showed that flagellin, the monomeric subunit of bacterial flagella, triggers innate immune responses mediated by Toll-like receptor 5. Here, we compared the effects of Salmonella enterica serovar Enteritidis lipopolysaccharide (LPS) and recombinant Salmonella enterica serovar Muenchen flagellin administered intravenously (100 microg) to mice. Flagellin and LPS both elicited a prototypical systemic inflammatory response, with increased levels of tumor necrosis factor alpha, gamma interferon, interleukin 6 and 10, and nitrate in plasma. Flagellin induced a widespread oxidative stress, evidenced by an increase in malondialdehyde and a decrease in reduced glutathione in most organs, as well as liver (increased plasma aminotransferases), but not renal, injury. Alternatively, LPS resulted in a less severe oxidative stress and triggered renal, but not liver, damage. Sequestration of polymorphonuclear neutrophils (increased myeloperoxidase activity) in the lungs was observed with both toxins, while only LPS recruited neutrophils in the gut. In additional experiments, the simultaneous administration of small doses of LPS and flagellin (10 microg) induced a synergistic enhancement of the production of proinflammatory cytokines. Our data support a novel concept implicating flagellin as a mediator of systemic inflammation, oxidant stress, and organ damage induced by gram-negative bacteria.
Mots-clé
Alanine Transaminase/blood Animals Aspartate Aminotransferases/blood Cytokines/blood Flagellin/*immunology Glutathione/metabolism Kidney/*injuries/metabolism Lipopolysaccharides/*immunology Liver/*injuries/metabolism Lung/metabolism Male Malondialdehyde/metabolism Mice Mice, Inbred BALB C Nitrates/blood Oxidative Stress/*immunology Peroxidase/metabolism Salmonella enterica/*immunology Salmonella enteritidis/*immunology Time Factors Urea/blood
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 18:00
Dernière modification de la notice
08/05/2019 21:44
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