Sialic acids on B cells are crucial for their survival and provide protection against apoptosis.

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State: Public
Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_8C01245BEAE4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sialic acids on B cells are crucial for their survival and provide protection against apoptosis.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Linder A.T., Schmidt M., Hitschfel J., Abeln M., Schneider P., Gerardy-Schahn R., Münster-Kühnel A.K., Nitschke L.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
21/06/2022
Peer-reviewed
Oui
Volume
119
Number
25
Pages
e2201129119
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Sialic acids (Sias) on the B cell membrane are involved in cell migration, in the control of the complement system and, as sialic acid-binding immunoglobulin-like lectin (Siglec) ligands, in the regulation of cellular signaling. We studied the role of sialoglycans on B cells in a mouse model with B cell-specific deletion of cytidine monophosphate sialic acid synthase (CMAS), the enzyme essential for the synthesis of sialoglycans. Surprisingly, these mice showed a severe B cell deficiency in secondary lymphoid organs. Additional depletion of the complement factor C3 rescued the phenotype only marginally, demonstrating a complement-independent mechanism. The B cell survival receptor BAFF receptor was not up-regulated, and levels of activated caspase 3 and processed caspase 8 were high in B cells of Cmas-deficient mice, indicating ongoing apoptosis. Overexpressed Bcl-2 could not rescue this phenotype, pointing to extrinsic apoptosis. These results show that sialoglycans on the B cell surface are crucial for B cell survival by counteracting several death-inducing pathways.
Keywords
Animals, Apoptosis, B-Cell Activation Factor Receptor/metabolism, B-Lymphocytes/physiology, Cell Survival, Gene Deletion, Mice, N-Acylneuraminate Cytidylyltransferase/genetics, Polysaccharides/metabolism, Sialic Acid Binding Immunoglobulin-like Lectins/metabolism, Sialic Acids/metabolism, B cell development, Siglec, extrinsic apoptosis, sialic acids
Pubmed
Web of science
Open Access
Yes
Create date
21/06/2022 12:22
Last modification date
11/05/2023 5:52
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