Sialic acids on B cells are crucial for their survival and provide protection against apoptosis.
Details
Serval ID
serval:BIB_8C01245BEAE4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sialic acids on B cells are crucial for their survival and provide protection against apoptosis.
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Publication state
Published
Issued date
21/06/2022
Peer-reviewed
Oui
Volume
119
Number
25
Pages
e2201129119
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Sialic acids (Sias) on the B cell membrane are involved in cell migration, in the control of the complement system and, as sialic acid-binding immunoglobulin-like lectin (Siglec) ligands, in the regulation of cellular signaling. We studied the role of sialoglycans on B cells in a mouse model with B cell-specific deletion of cytidine monophosphate sialic acid synthase (CMAS), the enzyme essential for the synthesis of sialoglycans. Surprisingly, these mice showed a severe B cell deficiency in secondary lymphoid organs. Additional depletion of the complement factor C3 rescued the phenotype only marginally, demonstrating a complement-independent mechanism. The B cell survival receptor BAFF receptor was not up-regulated, and levels of activated caspase 3 and processed caspase 8 were high in B cells of Cmas-deficient mice, indicating ongoing apoptosis. Overexpressed Bcl-2 could not rescue this phenotype, pointing to extrinsic apoptosis. These results show that sialoglycans on the B cell surface are crucial for B cell survival by counteracting several death-inducing pathways.
Keywords
Animals, Apoptosis, B-Cell Activation Factor Receptor/metabolism, B-Lymphocytes/physiology, Cell Survival, Gene Deletion, Mice, N-Acylneuraminate Cytidylyltransferase/genetics, Polysaccharides/metabolism, Sialic Acid Binding Immunoglobulin-like Lectins/metabolism, Sialic Acids/metabolism, B cell development, Siglec, extrinsic apoptosis, sialic acids
Pubmed
Web of science
Open Access
Yes
Create date
21/06/2022 12:22
Last modification date
11/05/2023 5:52