Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells

Détails

ID Serval
serval:BIB_8BF899F5DFF5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells
Périodique
Carcinogenesis
Auteur(s)
Applegate  L. A., Lautier  D., Frenk  E., Tyrrell  R. M.
ISSN
0143-3334 (Print)
Statut éditorial
Publié
Date de publication
1992
Volume
13
Numéro
9
Pages
1557-1560
Notes
DA - 19921029
LA - eng
PT - Journal Article
PT - Research Support, Non-U.S. Gov't
RN - 0 (Radiation-Sensitizing Agents)
RN - 1982-67-8 (Methionine Sulfoximine)
RN - 5072-26-4 (Buthionine Sulfoximine)
RN - 70-18-8 (Glutathione)
RN - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB - IM
Résumé
Spontaneous and induced mutations at the hypoxanthine guanine phosphoribosyl transferase locus have been measured in cultured human lymphoblastoid (TK6) cell populations under conditions in which cellular glutathione has been severely depleted by overnight treatment with buthionine-S,R-sulfoximine. At maximum levels of glutathione depletion, the increase in spontaneous frequency is at least 5-fold, a finding consistent with the possibility that cellular redox state can modulate the levels of pre-mutagenic damage arising as a result of normal metabolism in cultured human cells. Glutathione depletion does not lead to a significant enhancement in the frequency of mutants that arise as a result of irradiation at 313 nm but does lead to a 3-fold increase in mutations resulting from irradiation at 365 nm. These results indicate that glutathione may quench reactive intermediates that would otherwise lead to spontaneous mutations as well as a fraction of UVA radiation-induced premutagenic damage
Mots-clé
analogs & derivatives/Buthionine Sulfoximine/Cells/Cells,Cultured/deficiency/genetics/Glutathione/Humans/Hypoxanthine Phosphoribosyltransferase/metabolism/Methionine Sulfoximine/Mutation/pharmacology/physiology/Radiation-Sensitizing Agents/Ultraviolet Rays
Pubmed
Web of science
Création de la notice
18/02/2008 18:33
Dernière modification de la notice
03/03/2018 19:12
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