Ex vivo analysis of human antigen-specific CD8+ T-cell responses: quality assessment of fluorescent HLA-A2 multimer and interferon-gamma ELISPOT assays for patient immune monitoring.

Détails

ID Serval
serval:BIB_8BF0E4D9B030
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ex vivo analysis of human antigen-specific CD8+ T-cell responses: quality assessment of fluorescent HLA-A2 multimer and interferon-gamma ELISPOT assays for patient immune monitoring.
Périodique
Journal of Immunotherapy
Auteur(s)
Speiser D.E., Pittet M.J., Guillaume P., Lubenow N., Hoffman E., Cerottini J.C., Romero P.
ISSN
1524-9557, 1524-9557[linking]
Statut éditorial
Publié
Date de publication
2004
Volume
27
Numéro
4
Pages
298-308
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
The authors developed a standardized approach for immune monitoring of antigen-specific CD8+ T cells within peripheral blood lymphocytes (PBLs) that combines direct ex vivo analysis of Melan-A/MART-1 and influenza-specific CD8+ T cells with HLA-A2/peptide multimers and interferon-gamma ELISPOT assays. Here the authors assessed the quality of results obtained with 180 PBLs from healthy donors and melanoma patients. Reproducibility of the multimer assay was good (average of 15% variation). In the absence of in vivo antigen-specific T-cell responses, physiologic fluctuations of multimer-positive T cells was low, with variation coefficients of 20% for Melan-A and 28% for influenza-specific T cells. In contrast, patients with vaccination-induced T-cell responses had significantly increased T-cell frequencies clearly exceeding physiologic fluctuations. Comparable results were obtained with ELISPOT assays. In conclusion, this approach is well suited to assess T-cell responses as biologic endpoints in clinical vaccine studies.
Mots-clé
CD8-Positive T-Lymphocytes/immunology, Flow Cytometry, Fluorescence, HLA-A2 Antigen/blood, HLA-A2 Antigen/immunology, Humans, Influenza, Human/immunology, Interferon-gamma/blood, Interferon-gamma/immunology, Lymphocytes/immunology, Lymphocytes/metabolism, Melanoma/immunology, Melanoma/metabolism, Monitoring, Immunologic/methods, Peptides/immunology, Reproducibility of Results, Substrate Specificity, Vaccination
Pubmed
Web of science
Création de la notice
28/01/2008 12:14
Dernière modification de la notice
03/03/2018 19:12
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