In vivo mechanisms leading to transplantation tolerance induced by regulatory T cells

Détails

ID Serval
serval:BIB_8B95AA4599D1
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Titre
In vivo mechanisms leading to transplantation tolerance induced by regulatory T cells
Titre de la conférence
40th Annual Meeting Swiss Society of Nephrology
Auteur(s)
Golshayan D., Wyss J.C., Wyss C., Schaefer S., Lechler R., Lehr H.A., Pascual M.
Adresse
St. Gallen, Switzerland, December 3-5, 2008
ISBN
1424-7860
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
138
Série
Swiss Medical Weekly
Pages
6S
Langue
anglais
Notes
Publication type : Meeting Abstract
Résumé
Purpose: The mechanisms by which CD4+CD25+Foxp3+ T cells
(Tregs) regulate effector T cells in a transplantation setting and their in
vivo homeostasis still remain to be clarified. Using a mouse adoptive
transfer and skin transplantation model, we analyzed the in vivo
expansion, effector function and trafficking of effector T cells and
donor-specific Tregs, in response to an allograft.
Methods and materials: Antigen-specific Tregs were generated and
expanded in vitro by culturing freshly isolated Tregs from BALB/c
mice (H2d) with syngeneic dendritic cells pulsed with an allopeptide
(here the Kb peptide derived from the MHC class I molecule of
allogeneic H2b mice). Fluorescent-labelled CD4+CD25- naive T cells
and donor-antigen-specific Tregs were transferred alone or coinjected
into syngeneic BALB/c-Nude recipients transplanted with
allogeneic C57BL/6xBALB/c donor skin.
Results: As opposed to their in vitro hyporesponsiveness, Tregs
divided in vivo, migrated and accumulated in the allograft draining
lymph nodes (drLN) and within the graft. The co-transfer of Tregs did
not modify the early proliferation and homing of CD4+CD25- T cells
to secondary lymphoid organs. But, in the presence of Tregs, effector
T cells produced significantly less IFN- and IL-2 effector cytokines,
while higher amounts of IL-10 were detected in the spleen and drLN
of these mice. Furthermore, time-course studies showed that Tregs
were recruited into the allograft at a very early stage posttransplantation
and prevented infiltration by effector T cells.
Conclusion: Overall, our results suggest that suppression of graft
rejection involves the early recruitment of donor-specific Tregs at the
sites of antigenic challenge and that Tregs mainly regulate the effector
arm of T cell alloresponses.
Mots-clé
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Création de la notice
24/08/2010 15:52
Dernière modification de la notice
20/08/2019 14:50
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