Anti-idiotypic monoclonal antibody AB3, reacting with the primary antigen (CEA), can localize in human colon-carcinoma xenografts as efficiently as AB1.

Détails

ID Serval
serval:BIB_8B6B37BFA6CA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Anti-idiotypic monoclonal antibody AB3, reacting with the primary antigen (CEA), can localize in human colon-carcinoma xenografts as efficiently as AB1.
Périodique
International Journal of Cancer
Auteur(s)
de Moraes J.Z., Gesztesi J.L., Westermann P., Le Doussal J.M., Lopes J.D., Mach J.P.
ISSN
0020-7136 (Print)
ISSN-L
0020-7136
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
57
Numéro
4
Pages
586-591
Langue
anglais
Résumé
BALB/c mice were immunized with anti-idiotypic monoclonal (MAb) antibody (anti-Id or Ab2) directed against an AB1 MAb anti-carcinoembryonic (CEA) in order to obtain AB3 MAbs (anti-anti-Id). AB3 MAbs were shown to recognise the primary antigen (CEA) and one of them was tested extensively in vitro and in vivo. This AB3 MAb was shown to bind specifically to CEA on frozen sections of a human colon carcinoma by immunoperoxidase. Scatchard plot analyses showed that the affinity of this AB3 was of the same order of magnitude as the AB1. In vivo experiments, in nude mice bearing CEA-producing human colon-carcinoma xenografts showed that up to 30% of the intravenously injected dose of 125I-labelled AB3 were localized per gram of tumour tissue. Furthermore, calculation of the ratios of AB3 concentration in the tumour over those in normal organs such as lung, liver, kidney, spleen and bone gave relatively high values similar to results obtained with AB1. All together our results show that AB3 can localize as efficiently and specifically in the tumour as AB1, despite the fact that the mice from which it was derived were immunized with a mouse MAb (AB2) and had never been exposed to CEA.
Mots-clé
Animals, Antibodies, Anti-Idiotypic/immunology, Antibodies, Anti-Idiotypic/metabolism, Antibodies, Monoclonal/immunology, Antibodies, Monoclonal/metabolism, Antibody Specificity, Carcinoembryonic Antigen/analysis, Carcinoembryonic Antigen/immunology, Colonic Neoplasms/chemistry, Colonic Neoplasms/immunology, Humans, Immunization, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Transplantation, Tissue Distribution, Transplantation, Heterologous
Pubmed
Web of science
Création de la notice
18/11/2009 12:44
Dernière modification de la notice
03/03/2018 19:11
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